נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of the cis-acting elements controlling subgenomic mRNAs of Citrus tristeza virus: Production of positive- and negative-stranded 3′-terminal and positive-stranded 5′-terminal RNAs
Year:
2001
Source of publication :
Virology
Authors :
מוואסי, מוניר
;
.
Volume :
286
Co-Authors:
Gowda, S., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Satyanarayana, T., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Ayllón, M.A., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Albiach-Martí, M.R., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Mawassi, M., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Rabindran, S., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Garnsey, S.M., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Dawson, W.O., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Facilitators :
From page:
134
To page:
151
(
Total pages:
18
)
Abstract:
Citrus tristeza virus (CTV), a member of the Closteroviridae, has an ∼20-kb positive-sense RNA genome with two 5′ ORFs translated from the genomic RNA and 10 3′ genes expressed via nine or ten 3′-terminal subgenomic (sg) RNAs. The expression of the 3′ genes appears to have properties intermediate between the smaller viruses of the "alphavirus supergroup" and the larger viruses of the Coronaviridae. The sgRNAs are contiguous with the genome, without a common 5′ leader, and are associated with large amounts of complementary sgRNAs. Production of the different sgRNAs is regulated temporally and quantitatively, with the highly expressed genes having noncoding regions (NCR) 5′ of the ORFs. The cis-acting elements that control the highly expressed major coat protein (CP) gene and the intermediately expressed minor coat protein (CPm) gene were mapped and compared. Mutational analysis showed that the CP sgRNA controller element mapped within nts -47 to -5 upstream of the transcription start site, entirely within the NCR, while the CPm control region mapped within a 57 nt sequence within the upstream ORE Although both regions were predicted to fold into two stem-loop structures, mutagenesis suggested that primary structure might be more important than the secondary structure. Because each controller element produced large amounts of 3′-terminal positive- and negative-stranded sgRNAs, we could not differentiate whether the cis-acting element functioned as a promoter or terminator, or both. Reversal of the control element unexpectedly produced large amounts of a negative-stranded sgRNA apparently by termination of negative-stranded genomic RNA synthesis. Further examination of controller elements in their native orientation showed normal production of abundant amounts of positive-stranded sgRNAs extending to near the 5′-terminus, corresponding to termination at each controller element. Thus, each controller element produced three sgRNAs, a 5′-terminal positive strand and both positive- and negative-stranded 3′-terminal RNAs. Therefore, theoretically CTV could produce 30-33 species of RNAs in infected cells. © 2001 Academic Press.
Note:
Related Files :
animal cell
carboxy terminal sequence
chromosome mapping
cis acting element
Citrus tristeza virus
messenger rna synthesis
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/viro.2001.0987
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20026
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
You may also be interested in
Scientific Publication
Characterization of the cis-acting elements controlling subgenomic mRNAs of Citrus tristeza virus: Production of positive- and negative-stranded 3′-terminal and positive-stranded 5′-terminal RNAs
286
Gowda, S., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Satyanarayana, T., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Ayllón, M.A., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Albiach-Martí, M.R., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Mawassi, M., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Rabindran, S., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Garnsey, S.M., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Dawson, W.O., Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850, United States
Characterization of the cis-acting elements controlling subgenomic mRNAs of Citrus tristeza virus: Production of positive- and negative-stranded 3′-terminal and positive-stranded 5′-terminal RNAs
Citrus tristeza virus (CTV), a member of the Closteroviridae, has an ∼20-kb positive-sense RNA genome with two 5′ ORFs translated from the genomic RNA and 10 3′ genes expressed via nine or ten 3′-terminal subgenomic (sg) RNAs. The expression of the 3′ genes appears to have properties intermediate between the smaller viruses of the "alphavirus supergroup" and the larger viruses of the Coronaviridae. The sgRNAs are contiguous with the genome, without a common 5′ leader, and are associated with large amounts of complementary sgRNAs. Production of the different sgRNAs is regulated temporally and quantitatively, with the highly expressed genes having noncoding regions (NCR) 5′ of the ORFs. The cis-acting elements that control the highly expressed major coat protein (CP) gene and the intermediately expressed minor coat protein (CPm) gene were mapped and compared. Mutational analysis showed that the CP sgRNA controller element mapped within nts -47 to -5 upstream of the transcription start site, entirely within the NCR, while the CPm control region mapped within a 57 nt sequence within the upstream ORE Although both regions were predicted to fold into two stem-loop structures, mutagenesis suggested that primary structure might be more important than the secondary structure. Because each controller element produced large amounts of 3′-terminal positive- and negative-stranded sgRNAs, we could not differentiate whether the cis-acting element functioned as a promoter or terminator, or both. Reversal of the control element unexpectedly produced large amounts of a negative-stranded sgRNA apparently by termination of negative-stranded genomic RNA synthesis. Further examination of controller elements in their native orientation showed normal production of abundant amounts of positive-stranded sgRNAs extending to near the 5′-terminus, corresponding to termination at each controller element. Thus, each controller element produced three sgRNAs, a 5′-terminal positive strand and both positive- and negative-stranded 3′-terminal RNAs. Therefore, theoretically CTV could produce 30-33 species of RNAs in infected cells. © 2001 Academic Press.
Scientific Publication
You may also be interested in