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FEBS Letters
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Prujansky-Jakobovits, A., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
We describe a novel method for the isolation of human erythrocyte membranes based on the covalent binding of the cells to a solid support via cleavable disulfide bonds. After attachment, the cells are lysed, washed and the resultant membranes detached from the support by mild reduction with dithiothreitol. The application of this method to the isolation and identification of outer cell surface proteins is described.
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תנאי שימוש
Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support
101
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Prujansky-Jakobovits, A., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support
We describe a novel method for the isolation of human erythrocyte membranes based on the covalent binding of the cells to a solid support via cleavable disulfide bonds. After attachment, the cells are lysed, washed and the resultant membranes detached from the support by mild reduction with dithiothreitol. The application of this method to the isolation and identification of outer cell surface proteins is described.
Scientific Publication
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