אסיף מאגר המחקר החקלאי

Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support

Year:

1979

Source of publication :

Authors :

Volume :

101

Co-Authors:

Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Prujansky-Jakobovits, A., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel

Facilitators :

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Abstract:

We describe a novel method for the isolation of human erythrocyte membranes based on the covalent binding of the cells to a solid support via cleavable disulfide bonds. After attachment, the cells are lysed, washed and the resultant membranes detached from the support by mild reduction with dithiothreitol. The application of this method to the isolation and identification of outer cell surface proteins is described.

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