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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA
Year:
1978
Source of publication :
Molecular Biology Reports
Authors :
רוזנר, אריה
;
.
Volume :
4
Co-Authors:
Rosner, A., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Bastos, R.N., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Aviv, H., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
253
To page:
256
(
Total pages:
4
)
Abstract:
A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared. © 1978 Dr. W. Junk bv Publishers.
Note:
Related Files :
cycloserine
DNA, Recombinant
drug effect
Methods
Plasmid
Plasmids
Streptomyces griseus
tetracycline
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00777564
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20104
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
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Scientific Publication
Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA
4
Rosner, A., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Bastos, R.N., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Aviv, H., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA
A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared. © 1978 Dr. W. Junk bv Publishers.
Scientific Publication
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