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פותח על ידי קלירמאש פתרונות בע"מ -
Beyond aflatoxin: Four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters
Year:
2010
Source of publication :
Molecular Plant Pathology
Authors :
הורוביץ, סיגל בראון
;
.
Volume :
11
Co-Authors:
Georgianna, D.R., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States, Microbiology and Molecular Genetics Department, Duke University Medical Center, Durham, NC 27710, United States
Fedorova, N.D., J. Craig Venter Institute, Rockville, MD 20850, United States
Burroughs, J.L., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Dolezal, A.L., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Bok, J.W., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Horowitz-Brown, S., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Woloshuk, C.P., Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, United States
Yu, J., Southern Regional Research Center, US Department of Agriculture/Agricultural Research Service, New Orleans, LA 70124, United States
Keller, N.P., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Payne, G.A., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Facilitators :
From page:
213
To page:
226
(
Total pages:
14
)
Abstract:
Species of Aspergillus produce a diverse array of secondary metabolites, and recent genomic analysis has predicted that these species have the capacity to synthesize many more compounds. It has been possible to infer the presence of 55 gene clusters associated with secondary metabolism in Aspergillus flavus; however, only three metabolic pathways - aflatoxin, cyclopiazonic acid (CPA) and aflatrem - have been assigned to these clusters. To gain an insight into the regulation of and to infer the ecological significance of the 55 secondary metabolite gene clusters predicted in A. flavus, we examined their expression over 28 diverse conditions. Variables included culture medium and temperature, fungal development, colonization of developing maize seeds and misexpression of laeA, a global regulator of secondary metabolism. Hierarchical clustering analysis of expression profiles allowed us to categorize the gene clusters into four distinct clades. Gene clusters for the production of aflatoxins, CPA and seven other unknown compound(s) were identified as belonging to one clade. To further explore the relationships found by gene expression analysis, aflatoxin and CPA production were quantified under five different cell culture environments known to be conducive or nonconducive for aflatoxin biosynthesis and during the colonization of developing maize seeds. Results from these studies showed that secondary metabolism gene clusters have distinctive gene expression profiles. Aflatoxin and CPA were found to have unique regulation, but are sufficiently similar that they would be expected to co-occur in substrates colonized with A. flavus. © 2009 Blackwell Publishing Ltd.
Note:
Related Files :
Aspergillus
Genes, Fungal
Genetics
metabolism
Microbiology
multigene family
pH
temperature
Zea mays
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1364-3703.2009.00594.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20119
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
You may also be interested in
Scientific Publication
Beyond aflatoxin: Four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters
11
Georgianna, D.R., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States, Microbiology and Molecular Genetics Department, Duke University Medical Center, Durham, NC 27710, United States
Fedorova, N.D., J. Craig Venter Institute, Rockville, MD 20850, United States
Burroughs, J.L., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Dolezal, A.L., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Bok, J.W., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Horowitz-Brown, S., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Woloshuk, C.P., Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, United States
Yu, J., Southern Regional Research Center, US Department of Agriculture/Agricultural Research Service, New Orleans, LA 70124, United States
Keller, N.P., Department of Medical Microbiology and Immunology, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, United States
Payne, G.A., Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, United States
Beyond aflatoxin: Four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters
Species of Aspergillus produce a diverse array of secondary metabolites, and recent genomic analysis has predicted that these species have the capacity to synthesize many more compounds. It has been possible to infer the presence of 55 gene clusters associated with secondary metabolism in Aspergillus flavus; however, only three metabolic pathways - aflatoxin, cyclopiazonic acid (CPA) and aflatrem - have been assigned to these clusters. To gain an insight into the regulation of and to infer the ecological significance of the 55 secondary metabolite gene clusters predicted in A. flavus, we examined their expression over 28 diverse conditions. Variables included culture medium and temperature, fungal development, colonization of developing maize seeds and misexpression of laeA, a global regulator of secondary metabolism. Hierarchical clustering analysis of expression profiles allowed us to categorize the gene clusters into four distinct clades. Gene clusters for the production of aflatoxins, CPA and seven other unknown compound(s) were identified as belonging to one clade. To further explore the relationships found by gene expression analysis, aflatoxin and CPA production were quantified under five different cell culture environments known to be conducive or nonconducive for aflatoxin biosynthesis and during the colonization of developing maize seeds. Results from these studies showed that secondary metabolism gene clusters have distinctive gene expression profiles. Aflatoxin and CPA were found to have unique regulation, but are sufficiently similar that they would be expected to co-occur in substrates colonized with A. flavus. © 2009 Blackwell Publishing Ltd.
Scientific Publication
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