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פותח על ידי קלירמאש פתרונות בע"מ -
Synthesis of adipokinetic hormone (AKH-I) in the locust brain
Year:
1987
Source of publication :
Insect Biochemistry
Authors :
רפאלי, עדה
;
.
Volume :
17
Co-Authors:
Moshitzky, P., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Henzel, W.J., Genentech Inc., South San Francisco, CA 94080, United States
Rafaeli, A., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Ramachandran, J., Genentech Inc., South San Francisco, CA 94080, United States
Applebaum, S.W., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Facilitators :
From page:
1133
To page:
1137
(
Total pages:
5
)
Abstract:
Methanol extracts of vitellogenic female locust brains contain two factors that inhibit protein synthesis in fat body tissue excised from such individuals. One of these factors (BI) elicits lipid mobilization when injected into adult male locusts. The retention times of BI on an RP-18 column and on an RP-4 column are identical to those of synthetic locust adipokinetic hormone (AKH-I) on each of these columns. Half maximal inhibition of protein synthesis in excised adult locust fat bodies is exerted by 0.05 brain extract equivalents of BI, which is equivalent to activity elicited by 1.5 pmol of AKH-I, as previously determined by AKH-radioimmunoassay. Enzymatic hydrolysis of the N-terminal pyroglutamate, followed by amino acid sequence analysis, indicates that the structure of BI is similar to that of the decapeptide AKH-I synthesized in the glandular lobe of the locust corpora cardiaca (CC). Incorporation of [5-3H]tryptophan into BI of locust brains incubated in vitro indicates that the AKH-I present in the brain is synthesized in situ and is not transported from the CC. Similar incorporation of radiolabel into AKH-I is obtained when excised CC are incubated in vitro. © 1987.
Note:
Related Files :
brain AKH-I sequence
brain AKH-I synthesis
inhibition of protein synthesis
Locusta migratoria
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0020-1790(87)90084-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20141
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
Scientific Publication
Synthesis of adipokinetic hormone (AKH-I) in the locust brain
17
Moshitzky, P., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Henzel, W.J., Genentech Inc., South San Francisco, CA 94080, United States
Rafaeli, A., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Ramachandran, J., Genentech Inc., South San Francisco, CA 94080, United States
Applebaum, S.W., Department of Entomology, Faculty of Agriculture, The Hebrew University, Rehovot 76-100, Israel
Synthesis of adipokinetic hormone (AKH-I) in the locust brain
Methanol extracts of vitellogenic female locust brains contain two factors that inhibit protein synthesis in fat body tissue excised from such individuals. One of these factors (BI) elicits lipid mobilization when injected into adult male locusts. The retention times of BI on an RP-18 column and on an RP-4 column are identical to those of synthetic locust adipokinetic hormone (AKH-I) on each of these columns. Half maximal inhibition of protein synthesis in excised adult locust fat bodies is exerted by 0.05 brain extract equivalents of BI, which is equivalent to activity elicited by 1.5 pmol of AKH-I, as previously determined by AKH-radioimmunoassay. Enzymatic hydrolysis of the N-terminal pyroglutamate, followed by amino acid sequence analysis, indicates that the structure of BI is similar to that of the decapeptide AKH-I synthesized in the glandular lobe of the locust corpora cardiaca (CC). Incorporation of [5-3H]tryptophan into BI of locust brains incubated in vitro indicates that the AKH-I present in the brain is synthesized in situ and is not transported from the CC. Similar incorporation of radiolabel into AKH-I is obtained when excised CC are incubated in vitro. © 1987.
Scientific Publication
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