חיפוש מתקדם
Wang, X., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Donald Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, United States
Kobiler, I., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lichter, A., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Leikin-Frenkel, A., Minerva Center, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The preformed (Z,Z)-1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene (AFD) is the most active antifungal compound in avocado; it affects the quiescence nature of infection of unripe fruit by Colletotrichum gloeosporioides. Fatty acid elongase was hypothesized to participate in the biosynthesis of AFD, therefore the enzymatic activity of avocado fatty acid elongase (AVFAE) and the expression pattern of one of the genes encoding this enzyme were determined. Using avfae1 as a probe, high expression was detected in young fruits and leaves, those being the organs in which the incorporation of the AFD precursor, linoleic acid, and the level of AFD were the highest. In contrast, no expression of avfae1 could be detected in mature leaves and fruits and the content of AFD was lower. The expression of avfae1, the enzymatic activity of fatty acid elongase, and the level of AFD in unripe-resistant fruits increased transiently when the fruits were exposed to ethylene (40 μl/l), low temperature (4°C) or 1 mM H2O2, but ripe fruits were not affected. The present results suggest that the elongation of the linoleic acid is an important step in the biosynthesis of AFD, and hence, in the quiescence of C. gloeosporioides in unripe fruits. © 2005 Elsevier Ltd. All rights reserved.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Enhanced expression of avfae1 encoding a long-chain fatty acid elongase during the induction of the antifungal diene in avocado fruits
65
Wang, X., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Donald Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, United States
Kobiler, I., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lichter, A., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Leikin-Frenkel, A., Minerva Center, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Produce, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Enhanced expression of avfae1 encoding a long-chain fatty acid elongase during the induction of the antifungal diene in avocado fruits
The preformed (Z,Z)-1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene (AFD) is the most active antifungal compound in avocado; it affects the quiescence nature of infection of unripe fruit by Colletotrichum gloeosporioides. Fatty acid elongase was hypothesized to participate in the biosynthesis of AFD, therefore the enzymatic activity of avocado fatty acid elongase (AVFAE) and the expression pattern of one of the genes encoding this enzyme were determined. Using avfae1 as a probe, high expression was detected in young fruits and leaves, those being the organs in which the incorporation of the AFD precursor, linoleic acid, and the level of AFD were the highest. In contrast, no expression of avfae1 could be detected in mature leaves and fruits and the content of AFD was lower. The expression of avfae1, the enzymatic activity of fatty acid elongase, and the level of AFD in unripe-resistant fruits increased transiently when the fruits were exposed to ethylene (40 μl/l), low temperature (4°C) or 1 mM H2O2, but ripe fruits were not affected. The present results suggest that the elongation of the linoleic acid is an important step in the biosynthesis of AFD, and hence, in the quiescence of C. gloeosporioides in unripe fruits. © 2005 Elsevier Ltd. All rights reserved.
Scientific Publication
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