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Thirty-two natural elite stands of Pinus brutia Ten. subsp. brutia, used for seed production, were analyzed by starch gel isoenzyme electrophoresis, to determine their genetic diversity and structure. The average number of alleles per locus was 2.16, the range from 1.95 to 2.40
average percentage of polymorphic loci, at the 95 %criterion was 68 %, the range from 65 to 75 %
average effective number of alleles per locus was 1.49, the range from 1.41 to 1.60. The observed and expected heterozygosity were 0.191 (ranging from 0.144 to 0.267) and 0.271 (ranging from 0.233 to 0.312), respectively. Most of the genetic diversity (91 %) resides within populations. Inter-population gene flow according to Wright's formula was 2.55
the average gene flow between geographically grouped populations was 3.52, and that between latitudinally grouped populations was 2.81. There were significant linear regressions between allele frequencies in several loci among enzyme systems, and also between allele frequencies in several loci of different enzyme systems and the geographical variables of the stand (longitude, latitude, altitude, and slope aspect). The results imply that the formerly designated seed distribution zones should be reconsidered, because each seed stand is unique in its set of alleles, therefore, its seeds can be distributed only in the adjacent forest areas that have similar ecological features.
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