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פותח על ידי קלירמאש פתרונות בע"מ -
Modulated fatty acid desaturation via overexpression of two distinct ω-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants
Year:
2005
Source of publication :
Plant Journal
Authors :
בן חיים, גוזל
;
.
ברג, רבקה
;
.
דהן, ירדנה
;
.
זלץ, יחיעם
;
.
Volume :
44
Co-Authors:
Zhang, M., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel, College of Horticulture, Northwest Sci-Tech. University of Agriculture and Forestry, Yangling, Shaanxi 712100, China
Barg, R., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Yin, M., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Gueta-Dahan, Y., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Leikin-Frenkel, A., Minerva Center for Cholesterol Gallstones and Lipid Metabolism in Liver, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Salts, Y., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Shabtai, S., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Ben-Hayyim, G., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
361
To page:
371
(
Total pages:
11
)
Abstract:
Changes in the degree of fatty acid (FA) desaturation are implicated in plant responses to various abiotic stresses, including heat, salt and drought. However, it is still not known whether decreased levels of linolenic acid, found in many plants subjected to salt and drought stress, reflect a mechanism of defence or damage. We addressed this question by generating tobacco cells and plants ectopically overexpressing two FA desaturases: the cytosolic FAD3 or the plastidic FAD8. A remarkable increase in the ratio of total linolenic to linoleic acids resulted from overexpression of FAD3, whereas ectopic overexpression of FAD8 induced an increased ratio mainly in the plastidic lipids. Here we present evidence that overexpressing FAD8 imposes much greater heat sensitivity than does FAD3 overexpression, in both cultured cells and whole plants. Overexpression of either FAD3 or FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. These findings suggest that a drought-induced decreased level of linolenic acid reflects damage. Our results point to the potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance. © 2005 Blackwell Publishing Ltd.
Note:
Related Files :
enzymes
fatty acids
Genetics
heat stress
metabolism
omega 3 fatty acid desaturase
Plants
water
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2005.02536.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20188
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
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Scientific Publication
Modulated fatty acid desaturation via overexpression of two distinct ω-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants
44
Zhang, M., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel, College of Horticulture, Northwest Sci-Tech. University of Agriculture and Forestry, Yangling, Shaanxi 712100, China
Barg, R., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Yin, M., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Gueta-Dahan, Y., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Leikin-Frenkel, A., Minerva Center for Cholesterol Gallstones and Lipid Metabolism in Liver, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Salts, Y., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Shabtai, S., Institute of Field and Garden Crops, Volcani Center, Bet Dagan, Israel
Ben-Hayyim, G., Institute of Horticulture, ARO, Volcani Center, Bet Dagan, Israel
Modulated fatty acid desaturation via overexpression of two distinct ω-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants
Changes in the degree of fatty acid (FA) desaturation are implicated in plant responses to various abiotic stresses, including heat, salt and drought. However, it is still not known whether decreased levels of linolenic acid, found in many plants subjected to salt and drought stress, reflect a mechanism of defence or damage. We addressed this question by generating tobacco cells and plants ectopically overexpressing two FA desaturases: the cytosolic FAD3 or the plastidic FAD8. A remarkable increase in the ratio of total linolenic to linoleic acids resulted from overexpression of FAD3, whereas ectopic overexpression of FAD8 induced an increased ratio mainly in the plastidic lipids. Here we present evidence that overexpressing FAD8 imposes much greater heat sensitivity than does FAD3 overexpression, in both cultured cells and whole plants. Overexpression of either FAD3 or FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. These findings suggest that a drought-induced decreased level of linolenic acid reflects damage. Our results point to the potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance. © 2005 Blackwell Publishing Ltd.
Scientific Publication
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