אסיף מאגר המחקר החקלאי

Interaction of the bacteriocin pediocin SJ-1 with the cytoplasmic membrane of sensitive bacterial cells as detected by ANS fluorescence

Year:

1994

Source of publication :

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Volume :

76

Co-Authors:

Schved, F., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Lindner, P., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Juven, B.J., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel

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Abstract:

The 1-anilino-8-naphthalenesulphonic acid (ANS) fluorescent probe was used to monitor alterations in the cytoplasmic membrane of sensitive Lactobacillus plantarum cells, caused by pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. The addition of pediocin SJ-1 to the sensitive cells caused an increase in fluorescence intensity of ANS and a blue shift in its emission maximum from 520 to 475 nm. None of these spectral changes could be detected when pediocin SJ-1 was added to cells of a Lact. plantarum variant resistant to pediocin SJ-1. Upon the addition of pediocin SJ-1, dose- dependent energy transfer took place between tryptophanyl residues in the cytoplasmic membrane of sensitive cells and ANS. Similar ANS-fluorescence changes were observed with the bacteriocin nisin. The concentrations of pediocin SJ-1 needed to effect changes in the fluorescence spectrum of ANS were of the same magnitude as those required for a bactericidal effect and the release of u.v.-absorbing material. A hypothesis on the mode of action of pediocin SJ-1 is proposed.

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