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פותח על ידי קלירמאש פתרונות בע"מ -
Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTXR-cell line
Year:
1987
Source of publication :
Plant Molecular Biology
Authors :
ברג, רבקה
;
.
פרל, מאיר
;
.
Volume :
8
Co-Authors:
Barg, R., Department of Plant Genetics and Breeding, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Perl, M., Department of Medicinal and Spice Crops, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Beckmann, J.S., Department of Plant Genetics and Breeding, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
87
To page:
94
(
Total pages:
8
)
Abstract:
A petunia cell line, 1ECB, was previously isolated by the stepwise selection procedure, for resistance to methotrexate (MTX), an antimetabolite for the enzyme dihydrofolate reductase (DHFR). Using ammonium sulfate precipitates of cell lysates of cell line 1ECB and its parental cell line (WT), it was found that the mutant has an increase of 400 fold in 3H-MTX binding capacity and a decrease in the affinity for MTX binding, at two orders of magnitude, in comparison with the WT. In addition, the DHFR specific activity in the mutant increased only moderately (5- to 10-fold), this activity is extremely sensitive to MTX inhibition as compared to the WT. It is evident that the MTX resistance of line 1ECB results mainly from overproduction of an MTX-binding protein which differs from the WT DHFR by four biochemical criteria. This protein may serve as a trap for the excess amounts of MTX to which the cells are exposed. © 1987 Martinus Nijhoff Publishers.
Note:
Related Files :
amplification
DHFR
MTX-binding protein
MTX-resistance
petunia cell suspension
stepwise selection
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00016437
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20225
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
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Scientific Publication
Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTXR-cell line
8
Barg, R., Department of Plant Genetics and Breeding, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Perl, M., Department of Medicinal and Spice Crops, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Beckmann, J.S., Department of Plant Genetics and Breeding, Agricultural Research Organization, the Volcani Center, Bet Dagan, 50250, Israel
Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTXR-cell line
A petunia cell line, 1ECB, was previously isolated by the stepwise selection procedure, for resistance to methotrexate (MTX), an antimetabolite for the enzyme dihydrofolate reductase (DHFR). Using ammonium sulfate precipitates of cell lysates of cell line 1ECB and its parental cell line (WT), it was found that the mutant has an increase of 400 fold in 3H-MTX binding capacity and a decrease in the affinity for MTX binding, at two orders of magnitude, in comparison with the WT. In addition, the DHFR specific activity in the mutant increased only moderately (5- to 10-fold), this activity is extremely sensitive to MTX inhibition as compared to the WT. It is evident that the MTX resistance of line 1ECB results mainly from overproduction of an MTX-binding protein which differs from the WT DHFR by four biochemical criteria. This protein may serve as a trap for the excess amounts of MTX to which the cells are exposed. © 1987 Martinus Nijhoff Publishers.
Scientific Publication
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