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פותח על ידי קלירמאש פתרונות בע"מ -
Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: In vivo and ex vivo studies in mice
Year:
2007
Source of publication :
Theriogenology
Authors :
שלום, יבין
;
.
Volume :
67
Co-Authors:
Aroyo, A., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel, Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Yavin, S., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel, Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Roth, Z., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel
Facilitators :
From page:
1013
To page:
1021
(
Total pages:
9
)
Abstract:
Mammalian oocytes are susceptible to thermal stress at various stages of follicular development. We examined whether the ovarian pool of oocytes is susceptible to maternal hyperthermia and if so, whether hyperthermia at the germinal vesicle (GV) stage further affects the developmental competence of preimplantation embryos and offspring quality. Synchronized female mice were exposed to thermal stress (40 °C, 65% RH) for 1.5-2 h or maintained under normothermal conditions (25 °C, 45% RH). Thereafter, mice were paired with stud males. In the first experiment, mated mice were sacrificed 20 h post hCG administration, and in vivo-derived zygotes were recovered and cultured in vitro. Maternal hyperthermia decreased the percentage of putative zygotes of apparent normal morphology in the heat-stressed group (81 ± 1.3%) as compared to the control group (86 ± 1.2%). Developmental competence was also compromised as expressed by the disruption in cleavage timing pattern, resulting in a reduced developmental rate to the blastocyst stage (57 ± 2.6% versus 84 ± 1.9%). In the second experiment, both groups were left with stud males until litter delivery. Litter size in the first delivery cycle was lower for the heat-stressed group (7.7 ± 1.1 pups), followed by a slight increase throughout consecutive cycles as compared to the control group (11.3 ± 1.0 pups). Behavioral examinations of 8-week-old pups revealed similar locomotor activity and learning potential between the groups. In summary, the findings indicate that a subpopulation of the ovarian pool of follicles is highly sensitive to thermal stress and that maternal hyperthermia disrupts developmental competence of GV-stage oocytes. Pups that developed from oocytes that survived thermal stress exhibited a developmental potential similar to that of the of control pups. © 2006 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animal
Animals
Female
heat stress
Male
mice
pregnancy
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.theriogenology.2006.12.001
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20246
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:35
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Scientific Publication
Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: In vivo and ex vivo studies in mice
67
Aroyo, A., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel, Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Yavin, S., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel, Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Roth, Z., Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, POB 12, Rehovot, 76100, Israel
Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: In vivo and ex vivo studies in mice
Mammalian oocytes are susceptible to thermal stress at various stages of follicular development. We examined whether the ovarian pool of oocytes is susceptible to maternal hyperthermia and if so, whether hyperthermia at the germinal vesicle (GV) stage further affects the developmental competence of preimplantation embryos and offspring quality. Synchronized female mice were exposed to thermal stress (40 °C, 65% RH) for 1.5-2 h or maintained under normothermal conditions (25 °C, 45% RH). Thereafter, mice were paired with stud males. In the first experiment, mated mice were sacrificed 20 h post hCG administration, and in vivo-derived zygotes were recovered and cultured in vitro. Maternal hyperthermia decreased the percentage of putative zygotes of apparent normal morphology in the heat-stressed group (81 ± 1.3%) as compared to the control group (86 ± 1.2%). Developmental competence was also compromised as expressed by the disruption in cleavage timing pattern, resulting in a reduced developmental rate to the blastocyst stage (57 ± 2.6% versus 84 ± 1.9%). In the second experiment, both groups were left with stud males until litter delivery. Litter size in the first delivery cycle was lower for the heat-stressed group (7.7 ± 1.1 pups), followed by a slight increase throughout consecutive cycles as compared to the control group (11.3 ± 1.0 pups). Behavioral examinations of 8-week-old pups revealed similar locomotor activity and learning potential between the groups. In summary, the findings indicate that a subpopulation of the ovarian pool of follicles is highly sensitive to thermal stress and that maternal hyperthermia disrupts developmental competence of GV-stage oocytes. Pups that developed from oocytes that survived thermal stress exhibited a developmental potential similar to that of the of control pups. © 2006 Elsevier Inc. All rights reserved.
Scientific Publication
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