חיפוש מתקדם
Theoretical and Applied Genetics
Ben-Chaim, A., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Borovsky, Y., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel
Falise, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Mazourek, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Kang, B.-C., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Paran, I., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel
Jahn, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Pungency or "heat" found in Capsicum fruit results from the biosynthesis and accumulation of alkaloid compounds known as capsaicinoids in the dissepiment, placental tissue adjacent to the seeds. Pepper cultivars differ with respect to their level of pungency because of quantitative and qualitative variation in capsaicinoid content. We analyzed the segregation of three capsaicinoids: capsaicin, dihydrocapsaicin and nordihydrocapsaicin in an inter-specific cross between a mildly pungent Capsicum annuum 'NuMex RNaky' and the wild, highly pungent C. frutescens accession BG2814-6. F3 families were analyzed in three trials in California and in Israel and a dense molecular map was constructed comprised mostly of loci defined by simple sequence repeat (SSR) markers. Six QTL controlling capsaicinoid content were detected on three chromosomes. One gene from the capsaicinoid biosynthetic pathway, BCAT, and one random fruit EST, 3A2, co-localized with QTL detected in this study on chromosomes 3 and 4. Because one confounding factor in quantitative determination of capsaicinoid is fruit size, fruit weight measurements were taken in two trials. Two QTL controlling fruit weight were detected, however, they did not co-localize with QTL detected for capsaicinoid content. The major contribution to the phenotypic variation of capsaicinoid content (24-42% of the total variation) was attributed to a digenic interaction between a main-effect QTL, cap7.1, and a marker located on chromosome 2 that did not have a main effect on the trait. A second QTL, cap7.2 is likely to correspond to the QTL, cap, identified in a previous study as having pronounced influence on capsaicinoid content. © 2006 Springer-Verlag.
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תנאי שימוש
QTL analysis for capsaicinoid content in Capsicum
113
Ben-Chaim, A., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Borovsky, Y., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel
Falise, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Mazourek, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Kang, B.-C., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
Paran, I., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel
Jahn, M., Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY 14853, United States
QTL analysis for capsaicinoid content in Capsicum
Pungency or "heat" found in Capsicum fruit results from the biosynthesis and accumulation of alkaloid compounds known as capsaicinoids in the dissepiment, placental tissue adjacent to the seeds. Pepper cultivars differ with respect to their level of pungency because of quantitative and qualitative variation in capsaicinoid content. We analyzed the segregation of three capsaicinoids: capsaicin, dihydrocapsaicin and nordihydrocapsaicin in an inter-specific cross between a mildly pungent Capsicum annuum 'NuMex RNaky' and the wild, highly pungent C. frutescens accession BG2814-6. F3 families were analyzed in three trials in California and in Israel and a dense molecular map was constructed comprised mostly of loci defined by simple sequence repeat (SSR) markers. Six QTL controlling capsaicinoid content were detected on three chromosomes. One gene from the capsaicinoid biosynthetic pathway, BCAT, and one random fruit EST, 3A2, co-localized with QTL detected in this study on chromosomes 3 and 4. Because one confounding factor in quantitative determination of capsaicinoid is fruit size, fruit weight measurements were taken in two trials. Two QTL controlling fruit weight were detected, however, they did not co-localize with QTL detected for capsaicinoid content. The major contribution to the phenotypic variation of capsaicinoid content (24-42% of the total variation) was attributed to a digenic interaction between a main-effect QTL, cap7.1, and a marker located on chromosome 2 that did not have a main effect on the trait. A second QTL, cap7.2 is likely to correspond to the QTL, cap, identified in a previous study as having pronounced influence on capsaicinoid content. © 2006 Springer-Verlag.
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