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פותח על ידי קלירמאש פתרונות בע"מ -
The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation
Year:
1998
Source of publication :
Plant Molecular Biology
Authors :
בורד, שאול
;
.
דרובי, סמיר
;
.
חלוץ, עדו
;
.
לומניץ, אלה
;
.
לרס, אמנון
;
.
Volume :
36
Co-Authors:
Lers, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Droby, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Chalutz, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
847
To page:
856
(
Total pages:
10
)
Abstract:
Exposure of harvested grapefruit to UV-C (254 nm) irradiation was previously found to induce resistance against the green mold decay caused by Penicillium digitatum. In order to gain insight into the mechanism of this UV-induced resistance we initiated a study for isolation of genes induced during this process. Using the differential display method we cloned cDNA representing an mRNA which is accumulated in grapefruit peel upon UV irradiation. Sequence analysis revealed that this cDNA represents a gene encoding for an isoflavone reductase-like protein and was termed IRL (isoflavone reductase-like). The grapefruit IRL protein sequence has high homology also to a novel family of other isoflavone reductase-like proteins present in few non-legume plants and whose function is not clear yet. The UV dose, and time following it, which lead to maximal accumulation of the IRL transcript were found to be similar to those leading to maximal induced resistance. The expression of the IRL gene was demonstrated to be induced also by wounding and pathogen infection.
Note:
Related Files :
Base Sequence
fungi
grapefruits
Oxidoreductases
Postharvest
Ultraviolet Rays
UV irradiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1005996515602
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20312
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:35
Scientific Publication
The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation
36
Lers, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Droby, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Chalutz, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation
Exposure of harvested grapefruit to UV-C (254 nm) irradiation was previously found to induce resistance against the green mold decay caused by Penicillium digitatum. In order to gain insight into the mechanism of this UV-induced resistance we initiated a study for isolation of genes induced during this process. Using the differential display method we cloned cDNA representing an mRNA which is accumulated in grapefruit peel upon UV irradiation. Sequence analysis revealed that this cDNA represents a gene encoding for an isoflavone reductase-like protein and was termed IRL (isoflavone reductase-like). The grapefruit IRL protein sequence has high homology also to a novel family of other isoflavone reductase-like proteins present in few non-legume plants and whose function is not clear yet. The UV dose, and time following it, which lead to maximal accumulation of the IRL transcript were found to be similar to those leading to maximal induced resistance. The expression of the IRL gene was demonstrated to be induced also by wounding and pathogen infection.
Scientific Publication
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