Co-Authors:
Gollop, R., Department of Fruit Tree Breeding and Molecular Genetics, ARO, the Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Farhi, S., Department of Fruit Tree Breeding and Molecular Genetics, ARO, the Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Perl, A., Department of Fruit Tree Breeding and Molecular Genetics, ARO, the Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Abstract:
The control of expression of the leucoanthocyanidin dioxygenase (LDOX) gene was studied in grape. LDOX converts leucoanthocyanidins to anthocyanidins in the anthocyanins pathway. In the present study, the promoter of the ldox gene was cloned and fused to the uidA reporter gene, and the expression of this fusion was analyzed in transformed plants and in transformed red fruit cell suspensions. The ldox promoter-uidA gene fusion was expressed in all the plant organs. White light, calcium and sucrose induced the ldox gene expression. The induction by both light and calcium under light suggests the possible involvement of a UV receptors signal transduction pathway in the induction of the ldox gene. The induction of the ldox gene by light and by sucrose under light indicates a close interaction between the sucrose and the light signaling pathways. Analysis of the ldox promoter sequence revealed the existence of several putative DNA binding motifs. © 2001 Elsevier Science Ireland Ltd.
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