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פותח על ידי קלירמאש פתרונות בע"מ -
Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation
Year:
1993
Source of publication :
Nature Biotechnology
Authors :
גלילי, שמואל
;
.
Volume :
11
Co-Authors:
Perl, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel, Dept. Fruit Tree Breed. and Genet., Volcani Center, Bet-Dagan 50250, Israel
Galili, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Shaul, O., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Ben-Tzvi, I., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Galili, G., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
715
To page:
718
(
Total pages:
4
)
Abstract:
Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.
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DOI :
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Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20403
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:36
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Scientific Publication
Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation
11
Perl, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel, Dept. Fruit Tree Breed. and Genet., Volcani Center, Bet-Dagan 50250, Israel
Galili, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Shaul, O., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Ben-Tzvi, I., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Galili, G., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation
Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.
Scientific Publication
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