Co-Authors:
Gavish, H., Dept of Plant Genetics, Weizmann Inst. of Science, P.O.B. 26, Rehovot, 76100, Israel
Vardi, A., Inst. of Horticulture, Agricultural Research Organization, The Volcani Center, Bet-Dagan, 50250, Israel
Fluhr, R., Dept of Plant Genetics, Weizmann Inst. of Science, P.O.B. 26, Rehovot, 76100, Israel
Abstract:
Highly efficient synchronous embryogenesis was induced in suspension cultures of sour orange (Citrus aurantium L.) by a change in the carbon source of the growth medium from sucrose to glycerol. In liquid culture the embryos developed into globular structures during a three week period. Embryo development showed an absolute requirement for the continued presence of glycerol. The embryo cell cultures turned green in the light, but light did not affect the course of development. The profiles of soluble cellular protein extracts of embryo and proembryogenic (PEM) cells were very similar as judged by two‐dimensional polyacrylamide gel electrophoresis. However, major differences were detected in the profiles of extracellular proteins. PEM cells accumulated extracellular glycoproteins of 53–57 kDa mass. Upon subculture in glycerol containing medium, the accumulation of these proteins ceased within two days. Developing embryos accumulated at least 4 new extracellular polypeptides of 41–42 kDa mass. In addition to these polypeptides, stage specific peroxidases and proteases were found. The relatively extended duration and synchrony in which these early developmental events take place make Citrus cultures an especially useful system for the study of early events in plant embryogenesis. Copyright © 1991, Wiley Blackwell. All rights reserved
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