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Journal of Virological Methods
Mooijman, K.A., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
Bahar, M., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
Muniesa, M., Dept. Microbiologia, Fac. Biologia, Universitat De Barcelona, Av. Diagonal 645, Barcelona 08028, Spain
Havelaar, A.H., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
During the European project `Bacteriophages in bathing waters' (January 1996-June 1999), research was carried out to optimise the method for detection and enumeration of F-specific (RNA) phages in water. It was evaluated whether further optimisation would be possible/needed for the procedure as described in the standard method of the International Organisation for Standardisation (ISO) 10705-1. The research focused mainly on optimisation of the different steps for culturing the host strain WG49 Salmonella Typhimurium. It was concluded that all steps described in ISO 10705-1 are necessary and, if followed carefully, using a culture of host strain WG49 Salmonella Typhimurium of good quality, reliable results could be obtained for the enumeration of F-specific RNA phages. © 2002 Elsevier Science B.V. All rights reserved.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
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תנאי שימוש
Optimisation of ISO 10705-1 on enumeration of F-specific bacteriophages
103
Mooijman, K.A., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
Bahar, M., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
Muniesa, M., Dept. Microbiologia, Fac. Biologia, Universitat De Barcelona, Av. Diagonal 645, Barcelona 08028, Spain
Havelaar, A.H., National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection (MGB), P.O. Box 1, 3720 BA Bilthoven, Netherlands
Optimisation of ISO 10705-1 on enumeration of F-specific bacteriophages
During the European project `Bacteriophages in bathing waters' (January 1996-June 1999), research was carried out to optimise the method for detection and enumeration of F-specific (RNA) phages in water. It was evaluated whether further optimisation would be possible/needed for the procedure as described in the standard method of the International Organisation for Standardisation (ISO) 10705-1. The research focused mainly on optimisation of the different steps for culturing the host strain WG49 Salmonella Typhimurium. It was concluded that all steps described in ISO 10705-1 are necessary and, if followed carefully, using a culture of host strain WG49 Salmonella Typhimurium of good quality, reliable results could be obtained for the enumeration of F-specific RNA phages. © 2002 Elsevier Science B.V. All rights reserved.
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