Lozano, R., Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705, United States
Wilzer Jr., K.R., Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705, United States

After injection into male and female fifth-instar larvae of Manduca sexta, [14C]cholesterol was converted to C21 steroids, [14C]pregn-5-ene-3β, 20-diols. These metabolites were isolated from 8-day-old pupae and were identified by TLC, HPLC, and GC-MS as the C-20 isomers of pregnene-3β, 20-diol. They also were isolated from male and female meconium fluid (of 16-day-old pupae) following injection of [14C]cholesterol into 14-day-old pupae. © 1989.