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פותח על ידי קלירמאש פתרונות בע"מ -
The amino terminal region delimited by Met1 and Met37 is an integral part of the 32 kDa herbicide binding protein
Year:
1987
Source of publication :
Plant Molecular Biology
Authors :
איל, יורם
;
.
Volume :
8
Co-Authors:
Eyal, Y., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Goloubinoff, P., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Edelman, M., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
337
To page:
343
(
Total pages:
7
)
Abstract:
The 33.5 kDa precursor of the 32 kDa herbicide binding protein is encoded in the chloroplast by the psbA gene. Previous work on the gene and protein did not pinpoint the initiation site of translation in vivo. To resolve this issue, constructs containing full length, truncated or mutated psbA from Solanum nigrum were used as templates for in vitro transcription in an SP6-promoter directed system. The resulting RNAs were then translated in a reticulocyte lysate system. The SDS-PAGE and partial proteolysis patterns of the translation products demonstrate that constructs containing the first ATG codon (Met1) of the psbA open reading frame code for a full length precursor protein (33.5 kDa), irrespective of whether the second ATG codon (Met37) is replaced by site directed mutagenesis or not. However, a 5′ deleted gene, lacking the codon for Met1 but containing the codon for Met37, codes for a truncated 29 kDa protein. It is concluded that the 33.5 kDa precursor protein and its 32 kDa maturation product initiate at Met1 of the psbA open reading frame. © 1987 Martinus Nijhoff Publishers.
Note:
Related Files :
Chloroplasts
in vitro translation
site directed mutagenesis
translation initiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00021313
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20451
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:36
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Scientific Publication
The amino terminal region delimited by Met1 and Met37 is an integral part of the 32 kDa herbicide binding protein
8
Eyal, Y., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Goloubinoff, P., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Edelman, M., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
The amino terminal region delimited by Met1 and Met37 is an integral part of the 32 kDa herbicide binding protein
The 33.5 kDa precursor of the 32 kDa herbicide binding protein is encoded in the chloroplast by the psbA gene. Previous work on the gene and protein did not pinpoint the initiation site of translation in vivo. To resolve this issue, constructs containing full length, truncated or mutated psbA from Solanum nigrum were used as templates for in vitro transcription in an SP6-promoter directed system. The resulting RNAs were then translated in a reticulocyte lysate system. The SDS-PAGE and partial proteolysis patterns of the translation products demonstrate that constructs containing the first ATG codon (Met1) of the psbA open reading frame code for a full length precursor protein (33.5 kDa), irrespective of whether the second ATG codon (Met37) is replaced by site directed mutagenesis or not. However, a 5′ deleted gene, lacking the codon for Met1 but containing the codon for Met37, codes for a truncated 29 kDa protein. It is concluded that the 33.5 kDa precursor protein and its 32 kDa maturation product initiate at Met1 of the psbA open reading frame. © 1987 Martinus Nijhoff Publishers.
Scientific Publication
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