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פותח על ידי קלירמאש פתרונות בע"מ -
Self-custom-made SFP arrays for nonmodel organisms
Year:
2012
Source of publication :
Methods in Molecular Biology
Authors :
אופיר, רון
;
.
שרמן, עמיר
;
.
Volume :
815
Co-Authors:
Ophir, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Research Center, Bet Dagan 50250, Israel
Sherman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Research Center, Bet Dagan 50250, Israel
Facilitators :
From page:
39
To page:
47
(
Total pages:
9
)
Abstract:
Successful genetic mapping is dependent upon a high-density set of markers. Therefore, tools for high-throughput discovery of genetic variation are essential. The most abundant genetic marker is the single-nucleotide polymorphism (SNP). However, except for model organisms, genomic information is still limited. Although high-throughput genomic sequencing technologies are becoming relatively inexpensive, only low-throughput genetic markers are accessible (e.g., simple sequence repeats). The use of sequencing for the discovery and screening of high-density genetic variation in whole populations is still expensive. Alternatively, hybridization of genomic DNA (gDNA) on a reference (either genome or transcriptome) is an efficient approach for genetic screening without knowing the alleles in advance (Borevitz et al. Proc Natl Acad Sci USA 104:12057-12062). We describe a protocol for the design of probes for a high-throughput genetic-marker discovery microarray, termed single feature polymorphism (SFP) array. Starting with consensus cDNA sequences (UniGenes), we use OligoWiz to design T m-optimized 50-bp long oligonucleotide probes (Ophir et al. BMC Genomics 11:269, 2010). This design is similar to expression arrays and we point out the differences.
Note:
Related Files :
Base Sequence
chromosome map
chromosome mapping
crop
genetic markers
Genetics
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/978-1-61779-424-7_4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20482
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:36
Scientific Publication
Self-custom-made SFP arrays for nonmodel organisms
815
Ophir, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Research Center, Bet Dagan 50250, Israel
Sherman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Research Center, Bet Dagan 50250, Israel
Self-custom-made SFP arrays for nonmodel organisms
Successful genetic mapping is dependent upon a high-density set of markers. Therefore, tools for high-throughput discovery of genetic variation are essential. The most abundant genetic marker is the single-nucleotide polymorphism (SNP). However, except for model organisms, genomic information is still limited. Although high-throughput genomic sequencing technologies are becoming relatively inexpensive, only low-throughput genetic markers are accessible (e.g., simple sequence repeats). The use of sequencing for the discovery and screening of high-density genetic variation in whole populations is still expensive. Alternatively, hybridization of genomic DNA (gDNA) on a reference (either genome or transcriptome) is an efficient approach for genetic screening without knowing the alleles in advance (Borevitz et al. Proc Natl Acad Sci USA 104:12057-12062). We describe a protocol for the design of probes for a high-throughput genetic-marker discovery microarray, termed single feature polymorphism (SFP) array. Starting with consensus cDNA sequences (UniGenes), we use OligoWiz to design T m-optimized 50-bp long oligonucleotide probes (Ophir et al. BMC Genomics 11:269, 2010). This design is similar to expression arrays and we point out the differences.
Scientific Publication
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