נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The Pun1 gene for pungency in pepper encodes a putative acyltransferase
Year:
2005
Source of publication :
Plant Journal
Authors :
פארן, אילן
;
.
Volume :
42
Co-Authors:
Stewart Jr., C., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Kang, B.-C., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Liu, K., National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, Hubei Province, China
Mazourek, M., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Moore, S.L., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Eun, Y.Y., Center for Plant Molecular Genetics and Breeding Research, Seoul National University, San 56-1, Shilllm 9-dong, Kwanak-gu, Seoul 151-742, South Korea
Kim, B.-D., Center for Plant Molecular Genetics and Breeding Research, Seoul National University, San 56-1, Shilllm 9-dong, Kwanak-gu, Seoul 151-742, South Korea
Paran, I., Department of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Jahn, M.M., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Facilitators :
From page:
675
To page:
688
(
Total pages:
14
)
Abstract:
Pungency in Capsicum fruits is due to the accumulation of the alkaloid capsaicin and its analogs. The biosynthesis of capsaicin is restricted to the genus Capsicum and results from the acylation of an aromatic moiety, vanillylamine, by a branched-chain fatty acid. Many of the enzymes involved in capsaicin biosynthesis are not well characterized and the regulation of the pathway is not fully understood. Based on the current pathway model, candidate genes were identified in public databases and the literature, and genetically mapped. A published EST co-localized with the Pun1 locus which is required for the presence of capsaicinoids. This gene, AT3, has been isolated and its nucleotide sequence has been determined in an array of genotypes within the genus. AT3 showed significant similarity to acyltransferases in the BAHD superfamily. The recessive allele at this locus contains a deletion spanning the promoter and first exon of the predicted coding region in every non-pungent accession tested. Transcript and protein expression of AT3 was tissue-specific and developmentally regulated. Virus-induced gene silencing of AT3 resulted in a decrease in the accumulation of capsaicinoids, a phenotype consistent with pun1. In conclusion, gene mapping, allele sequence data, expression profile and silencing analysis collectively indicate that the Pun1 locus in pepper encodes a putative acyltransferase, and the pun1 allele, used in pepper breeding for nearly 50 000 years, results from a large deletion at this locus. © 2005 Blackwell Publishing Ltd.
Note:
Related Files :
biosynthesis
enzymes
fatty acids
Fruits
Genes
genetic engineering
Genetics
metabolism
molecular genetics
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2005.02410.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20559
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:37
Scientific Publication
The Pun1 gene for pungency in pepper encodes a putative acyltransferase
42
Stewart Jr., C., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Kang, B.-C., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Liu, K., National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, Hubei Province, China
Mazourek, M., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Moore, S.L., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Eun, Y.Y., Center for Plant Molecular Genetics and Breeding Research, Seoul National University, San 56-1, Shilllm 9-dong, Kwanak-gu, Seoul 151-742, South Korea
Kim, B.-D., Center for Plant Molecular Genetics and Breeding Research, Seoul National University, San 56-1, Shilllm 9-dong, Kwanak-gu, Seoul 151-742, South Korea
Paran, I., Department of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Jahn, M.M., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
The Pun1 gene for pungency in pepper encodes a putative acyltransferase
Pungency in Capsicum fruits is due to the accumulation of the alkaloid capsaicin and its analogs. The biosynthesis of capsaicin is restricted to the genus Capsicum and results from the acylation of an aromatic moiety, vanillylamine, by a branched-chain fatty acid. Many of the enzymes involved in capsaicin biosynthesis are not well characterized and the regulation of the pathway is not fully understood. Based on the current pathway model, candidate genes were identified in public databases and the literature, and genetically mapped. A published EST co-localized with the Pun1 locus which is required for the presence of capsaicinoids. This gene, AT3, has been isolated and its nucleotide sequence has been determined in an array of genotypes within the genus. AT3 showed significant similarity to acyltransferases in the BAHD superfamily. The recessive allele at this locus contains a deletion spanning the promoter and first exon of the predicted coding region in every non-pungent accession tested. Transcript and protein expression of AT3 was tissue-specific and developmentally regulated. Virus-induced gene silencing of AT3 resulted in a decrease in the accumulation of capsaicinoids, a phenotype consistent with pun1. In conclusion, gene mapping, allele sequence data, expression profile and silencing analysis collectively indicate that the Pun1 locus in pepper encodes a putative acyltransferase, and the pun1 allele, used in pepper breeding for nearly 50 000 years, results from a large deletion at this locus. © 2005 Blackwell Publishing Ltd.
Scientific Publication
You may also be interested in