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Nematologica
Glazer, I., Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel
Kozodoi, E., Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel
Hashmi, G., Department of Entomology, Rutgers University, New Brunswick, NJ 08903, United States
Gaugler, R., Department of Entomology, Rutgers University, New Brunswick, NJ 08903, United States
Entomopathogenic nematode isolate (IS-5), from under the canopy of fruit trees in the arid 'Negev' region of Israel was found to be highly tolerant to temperatures above 30°C. Morphological measurements did not distinguish this isolate from Heterorhabditis bacteriophora Poinar, 1976. However, DNA extracted from four different genotypes (Heterorhabditis sp. IS-5, HP88 and IS-3 and H. megidis HSH2) amplified with six random primers yielded products from which it was calculated that the similarity between IS-5 and HP88 was 20%, between IS-3 and HP88 85% and between IS-3 and H. megidis 15.6%. Two hours at 37°C killed 79% HP88 infective juveniles but only 18% IS-5. Eight hours at 37°C killed 87% of HP88 but only 28% of IS-5. One hour at 40°C killed all infective juveniles of the HP88 isolate but only 26% of IS-5. Heat shock treatment for 2 h at 37°C before exposure to 40°C enhanced the survival of IS-5 juveniles to 43% as compared with non heat-shocked control. At 25°C IS-5 caused 100% mortality of Galleria mellonella larvae after 36 h and HP88 after 48 h. At 30°C IS-5 caused 100% mortality in 24 h. The average number of nematodes in cadavers exposed to IS-5 and HP88 for 48 h ranged from 15 to 16.3 and 7.3 to 10.8, respectively. At 25°C both these heterorhabditid isolates produced an average of 90,000 infective juveniles per cadaver. At 30°C and 33°C yields of IS-5 increased by 10% but those of HP88 reduced by 90%. At each temperature IS-5 juveniles emerged 2-3 days earlier than HP88.

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Biological characteristics of the entomopathogenic nematode Heterorhabditis sp. IS-5: A heat tolerant isolate from Israel
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Glazer, I., Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel
Kozodoi, E., Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel
Hashmi, G., Department of Entomology, Rutgers University, New Brunswick, NJ 08903, United States
Gaugler, R., Department of Entomology, Rutgers University, New Brunswick, NJ 08903, United States
Biological characteristics of the entomopathogenic nematode Heterorhabditis sp. IS-5: A heat tolerant isolate from Israel
Entomopathogenic nematode isolate (IS-5), from under the canopy of fruit trees in the arid 'Negev' region of Israel was found to be highly tolerant to temperatures above 30°C. Morphological measurements did not distinguish this isolate from Heterorhabditis bacteriophora Poinar, 1976. However, DNA extracted from four different genotypes (Heterorhabditis sp. IS-5, HP88 and IS-3 and H. megidis HSH2) amplified with six random primers yielded products from which it was calculated that the similarity between IS-5 and HP88 was 20%, between IS-3 and HP88 85% and between IS-3 and H. megidis 15.6%. Two hours at 37°C killed 79% HP88 infective juveniles but only 18% IS-5. Eight hours at 37°C killed 87% of HP88 but only 28% of IS-5. One hour at 40°C killed all infective juveniles of the HP88 isolate but only 26% of IS-5. Heat shock treatment for 2 h at 37°C before exposure to 40°C enhanced the survival of IS-5 juveniles to 43% as compared with non heat-shocked control. At 25°C IS-5 caused 100% mortality of Galleria mellonella larvae after 36 h and HP88 after 48 h. At 30°C IS-5 caused 100% mortality in 24 h. The average number of nematodes in cadavers exposed to IS-5 and HP88 for 48 h ranged from 15 to 16.3 and 7.3 to 10.8, respectively. At 25°C both these heterorhabditid isolates produced an average of 90,000 infective juveniles per cadaver. At 30°C and 33°C yields of IS-5 increased by 10% but those of HP88 reduced by 90%. At each temperature IS-5 juveniles emerged 2-3 days earlier than HP88.
Scientific Publication
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