נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Comparative analysis of mitochondrial citrate synthase gene structure, transcript level and enzymatic activity in acidless and acid-containing Citrus varieties
Year:
2001
Authors :
אור, אתי
;
.
דהן, אסתר
;
.
צדקה, אבי
;
.
Volume :
28
Co-Authors:
Sadka, A., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dahan, E., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Or, E., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Roose, M.L., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Marsh, K.B., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Cohen, L., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
383
To page:
390
(
Total pages:
8
)
Abstract:
Most citrus (Citrus L. spp.) fruits accumulate a considerable amount of citric acid in the vacuoles of the juice sac cells. As part of research aimed to understand the mechanism of acid accumulation, we compared the gene structures and transcript levels of citrate synthase in sour lemon (high acid, C. limon (L.) Burm.), 'Shamouti' orange (moderate acid, C. sinensis (L.) Osbeck) and sweet lime (acidless, C. limmetioides Tan.). Southern analyses suggested that a single gene for citrate synthase was present in the genomes of all three Citrus varieties. The gene structures seemed to be very similar, with minor differences in Shamouti orange. Overall, the transcript levels of citrate synthase were similar in sweet lime and sour lemon, and about 2-fold lower in Shamouti orange. The enzymatic activity of citrate synthase was compared between sour lemon and sweet lime. In sour lemon, the specific activity of the enzyme was induced early in fruit development and, in parallel with the increase in acid content, reached a maximal level, and did not diminish significantly towards fruit maturation; the pattern and level of activity detected during sweet lime fruit development were similar. These results suggest that the difference in acid accumulation between acidless and acid-containing fruits may not be attributed to changes in the activity of citrate synthase.
Note:
Related Files :
Cells
Citrus limettioides
Citrus limmetioides
Citrus limon
Citrus sinensis
enzymes
fruit acidity
Fruits
Genes
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20612
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:37
Scientific Publication
Comparative analysis of mitochondrial citrate synthase gene structure, transcript level and enzymatic activity in acidless and acid-containing Citrus varieties
28
Sadka, A., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dahan, E., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Or, E., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Roose, M.L., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Marsh, K.B., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Cohen, L., Institute of Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Comparative analysis of mitochondrial citrate synthase gene structure, transcript level and enzymatic activity in acidless and acid-containing Citrus varieties
Most citrus (Citrus L. spp.) fruits accumulate a considerable amount of citric acid in the vacuoles of the juice sac cells. As part of research aimed to understand the mechanism of acid accumulation, we compared the gene structures and transcript levels of citrate synthase in sour lemon (high acid, C. limon (L.) Burm.), 'Shamouti' orange (moderate acid, C. sinensis (L.) Osbeck) and sweet lime (acidless, C. limmetioides Tan.). Southern analyses suggested that a single gene for citrate synthase was present in the genomes of all three Citrus varieties. The gene structures seemed to be very similar, with minor differences in Shamouti orange. Overall, the transcript levels of citrate synthase were similar in sweet lime and sour lemon, and about 2-fold lower in Shamouti orange. The enzymatic activity of citrate synthase was compared between sour lemon and sweet lime. In sour lemon, the specific activity of the enzyme was induced early in fruit development and, in parallel with the increase in acid content, reached a maximal level, and did not diminish significantly towards fruit maturation; the pattern and level of activity detected during sweet lime fruit development were similar. These results suggest that the difference in acid accumulation between acidless and acid-containing fruits may not be attributed to changes in the activity of citrate synthase.
Scientific Publication
You may also be interested in