אסיף מאגר המחקר החקלאי

Occurrence, distribution and balance of ROS during nitrogen deprivation and early development in Colletotrichum acutatum infecting strawberry

Year:

2009

Source of publication :

Authors :

Volume :

842

Co-Authors:

Brown, S.H., Dept. of Plant Pathology and Microbiology, Hebrew University of Jerusalem, Rehovot 76100, Israel, Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Dept. of Plant Pathology and Microbiology, Hebrew University of Jerusalem, Rehovot 76100, Israel
Gollop, N., Dept. of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Chen, S., Dept. of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel

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Abstract:

A nonpathogenic mutant of Colletotrichum acutatum exhibited extended germ tube growth prior to appressoria formation on solid surface and strawberry leaves in the absence of an external nitrogen source, but grew necrotrophically when inoculated directly onto wounded sites. The deduced amino acid sequence of the impaired gene product was characterized as a transcriptional regulator of nitrogen metabolism. Inoculation of leaves with wildtype or the mutant in the presence of a preferred nitrogen source resulted in massive epiphytic hyphal production, appressoria formation and rapid symptom development. Proteins patterns of different developmental stages and growth conditions revealed variation in abundance of proteins belonging to the ROS (Reactive oxygen species) scavenger machinery. CuZn superoxide dismutase and glutathione peroxidase were upregulated at the appressoria formation stage and nitrogen limiting conditions, compared to growth in complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressoria formation stage. Fungal ROS was detected within germinating conidia during strawberry host prepenetration, penetration and colonization stages. Application of an exogenous antioxidant quenched ROS production and reduced appressoria formation frequency. This study indicates that developmental processes occurring under nutritional deprivation in C. acutatum infecting strawberry are facilitated by metabolic shifts which may be mediated by fungal ROS production.

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