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פותח על ידי קלירמאש פתרונות בע"מ -
Resumption of cyclin b and histone h1 kinase activity marks reproductive bud break in pear grown in the hot climate of Israel
Year:
2005
Source of publication :
Acta Horticulturae
Authors :
ברייר, יובל
;
.
פליישמן, משה
;
.
שרגל, עמיחי
;
.
Volume :
671
Co-Authors:
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Brayer, Y., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Shargal, A., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Grafi, G., Department of Plant Sciences, Weizman Institute of Science, Rehovot, Israel
Facilitators :
From page:
275
To page:
281
(
Total pages:
7
)
Abstract:
To compensate for the lack of chilling requirements of fruit trees grown in relatively hot climate, chemical treatments to break bud dormancy have to be applied. The success of such treatments depends on the identification of the proper stage of dormancy development. To date, no reliable means exists to indicate bud developmental stages in the orchard. We investigated the relationship between winter dormancy development and cell cycle regulation in the low chill requiring pear cultivar Spadona by a combination of morphological, histological and biochemical methods. The study was conducted in two orchards located in different climatic regions at 40 and 700 m above sea level. In both orchards, bud development was arrested during winter endodormancy. Fresh weight of mixed buds, pistil length and pedicel width increased toward the end of endodormancy. A sequence of flow cytometry analyses during endodormancy and its release in both orchards showed the existence of G2 nuclei correlated with phenotypic changes in the developing mixed buds. To find molecular markers anticipating bud break, we followed the seasonal expression of cyclin B and histone H1 kinase activity. In both orchards, mixed buds showed an increase in histone H1 kinase activity as well as in cyclin B levels just prior to the end of endodormancy. We suggest that the expression level of cyclin B and the activity of histone H1 kinase can serve as markers for early stages of mixed bud break in pear and probably in other species as well.
Note:
Related Files :
Bud dormancy
cell cycle
Chilling
Endodormancy
gene expression
Pyrus communis
Treedormancy
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
20639
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
You may also be interested in
Scientific Publication
Resumption of cyclin b and histone h1 kinase activity marks reproductive bud break in pear grown in the hot climate of Israel
671
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Brayer, Y., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Shargal, A., Department of Fruit Trees, Institute of Horticulture, ARO the Volcani Center, P.O. Box 6, Bet Dagan, Israel
Grafi, G., Department of Plant Sciences, Weizman Institute of Science, Rehovot, Israel
Resumption of cyclin b and histone h1 kinase activity marks reproductive bud break in pear grown in the hot climate of Israel
To compensate for the lack of chilling requirements of fruit trees grown in relatively hot climate, chemical treatments to break bud dormancy have to be applied. The success of such treatments depends on the identification of the proper stage of dormancy development. To date, no reliable means exists to indicate bud developmental stages in the orchard. We investigated the relationship between winter dormancy development and cell cycle regulation in the low chill requiring pear cultivar Spadona by a combination of morphological, histological and biochemical methods. The study was conducted in two orchards located in different climatic regions at 40 and 700 m above sea level. In both orchards, bud development was arrested during winter endodormancy. Fresh weight of mixed buds, pistil length and pedicel width increased toward the end of endodormancy. A sequence of flow cytometry analyses during endodormancy and its release in both orchards showed the existence of G2 nuclei correlated with phenotypic changes in the developing mixed buds. To find molecular markers anticipating bud break, we followed the seasonal expression of cyclin B and histone H1 kinase activity. In both orchards, mixed buds showed an increase in histone H1 kinase activity as well as in cyclin B levels just prior to the end of endodormancy. We suggest that the expression level of cyclin B and the activity of histone H1 kinase can serve as markers for early stages of mixed bud break in pear and probably in other species as well.
Scientific Publication
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