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פותח על ידי קלירמאש פתרונות בע"מ -
Regulatory interactions between quorum-sensing, Auxin, cytokinin, and the Hrp regulon in relation to gall formation and epiphytic fitness of pantoea agglomerans pv. gypsophilae
Year:
2009
Authors :
מנוליס-ששון, שולמית
;
.
צ'לופוביץ, לאורה
;
.
Volume :
22
Co-Authors:
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Panijel, M., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
849
To page:
856
(
Total pages:
8
)
Abstract:
Gall formation by Pantoea agglomérons pv. gypsophilae is controlled by hrp/hrc genes, phytohormones, and the quorum-sensing (QS) regulatory system. The interactions between these three components were investigated. Disruption of the QS genes pagl and pagR and deletion of both substantially reduced the transcription levels of the hrp regulatory genes hrpXY, hrpS, and hrpL, as determined by quantitative reverse-transcriptase polymerase chain reaction. Expression of hrpL in planta was inhibited by addition of 20 IiM or higher concentrations of the QS signal C4-HSL. The pagR and hrpL mutants caused an equivalent reduction of 1.3 orders in bacterial multiplication on bean leaves, suggesting possible mediation of the QS effect on epiphytic fitness of P. agglomerans pv. gypsophilae by the hrp regulatory system, indole-3-acetic acid (IAA) and cytokinin significantly affected the expression of the QS and hrp regulatory genes. Transcription of pagl, pagR, hrpL, and hrpS in planta was substantially reduced in iaaH mutant (disrupted in IAA biosynthesis via the indole-3-acetamide pathway) and etz mutant (disrupted in cytokinin biosynthesis). In contrast, the ipdC mutant (disrupted in IAA biosynthesis via the indole-3-pyruvate pathway) substantially increased expression of pagl, pagR, hrpL, and hrpS. Results presented suggest the involvement of IAA and cytokinins in regulation of the QS system and hrp regulatory genes. © 2009 The American Phytopathological Society.
Note:
Related Files :
cytokinins
Genetics
Growth, Development and Aging
metabolism
mutation
quorum sensing
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-22-7-0849
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20642
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
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Scientific Publication
Regulatory interactions between quorum-sensing, Auxin, cytokinin, and the Hrp regulon in relation to gall formation and epiphytic fitness of pantoea agglomerans pv. gypsophilae
22
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Panijel, M., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, 50250, Israel
Regulatory interactions between quorum-sensing, Auxin, cytokinin, and the Hrp regulon in relation to gall formation and epiphytic fitness of pantoea agglomerans pv. gypsophilae
Gall formation by Pantoea agglomérons pv. gypsophilae is controlled by hrp/hrc genes, phytohormones, and the quorum-sensing (QS) regulatory system. The interactions between these three components were investigated. Disruption of the QS genes pagl and pagR and deletion of both substantially reduced the transcription levels of the hrp regulatory genes hrpXY, hrpS, and hrpL, as determined by quantitative reverse-transcriptase polymerase chain reaction. Expression of hrpL in planta was inhibited by addition of 20 IiM or higher concentrations of the QS signal C4-HSL. The pagR and hrpL mutants caused an equivalent reduction of 1.3 orders in bacterial multiplication on bean leaves, suggesting possible mediation of the QS effect on epiphytic fitness of P. agglomerans pv. gypsophilae by the hrp regulatory system, indole-3-acetic acid (IAA) and cytokinin significantly affected the expression of the QS and hrp regulatory genes. Transcription of pagl, pagR, hrpL, and hrpS in planta was substantially reduced in iaaH mutant (disrupted in IAA biosynthesis via the indole-3-acetamide pathway) and etz mutant (disrupted in cytokinin biosynthesis). In contrast, the ipdC mutant (disrupted in IAA biosynthesis via the indole-3-pyruvate pathway) substantially increased expression of pagl, pagR, hrpL, and hrpS. Results presented suggest the involvement of IAA and cytokinins in regulation of the QS system and hrp regulatory genes. © 2009 The American Phytopathological Society.
Scientific Publication
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