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פותח על ידי קלירמאש פתרונות בע"מ -
The identification of a gene (Cwp1), silenced during Solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit
Year:
2007
Source of publication :
Plant Journal
Authors :
חובב, רן
;
.
מוי, מיכל
;
.
צ'חנובסקי, נועם
;
.
שפר, ארתור
;
.
Volume :
52
Co-Authors:
Hovav, R., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel, Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, IA 50011, United States
Chehanovsky, N., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
Moy, M., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
Jetter, R., Departments of Botany and Chemistry, University of British Columbia, Vancouver, BC, Canada
Schaffer, A.A., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
627
To page:
639
(
Total pages:
13
)
Abstract:
One of the most intriguing phenomena of fleshy fruit is the ability to maintain high water content at maturity, even following harvest. This is accomplished by a fruit cuticle that is highly impermeable to water diffusion. In this paper, we report on a novel genotype of tomato, developed via introgression from the wild species Solanum habrochaites, which is characterized by microfissuring of the fruit cuticle and dehydration of the mature fruit. The microfissure/dehydration phenotype is inherited as a single gene, termed Cwp1 (cuticular water permeability). The gene was fine mapped, and its identity was determined by map-based cloning and differential expression analysis in near-isogenic lines. Causality of the Cwp1 gene was shown by the heterologous transgenic expression of the gene in the cultivated tomato, which caused a microfissured fruit cuticle leading to dehydrated fruit. Cwp1 encodes for a protein of unidentified function in the DUF833 domain family. The gene is expressed in the fruit epidermis of the dehydrating genotype harbouring the wild-species introgression, but not in the cultivated tomato. It is expressed only in the primitive green-fruited wild tomato species, but is not expressed in the cultivated Solanum lycopersicum and the closely related Solanum cheesmaniae and Solanum pimpinellifolium, indicating a pre-adaptive role for Cwp1 silencing in the evolution and domestication of the cultivated tomato. © 2007 The Authors.
Note:
Related Files :
cloning
gene expression
Genetics
metabolism
molecular genetics
Solanum
water
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2007.03265.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20650
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
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Scientific Publication
The identification of a gene (Cwp1), silenced during Solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit
52
Hovav, R., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel, Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, IA 50011, United States
Chehanovsky, N., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
Moy, M., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
Jetter, R., Departments of Botany and Chemistry, University of British Columbia, Vancouver, BC, Canada
Schaffer, A.A., Institute of Field and Garden Crops, ARO, Volcani Center, Bet Dagan 50250, Israel
The identification of a gene (Cwp1), silenced during Solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit
One of the most intriguing phenomena of fleshy fruit is the ability to maintain high water content at maturity, even following harvest. This is accomplished by a fruit cuticle that is highly impermeable to water diffusion. In this paper, we report on a novel genotype of tomato, developed via introgression from the wild species Solanum habrochaites, which is characterized by microfissuring of the fruit cuticle and dehydration of the mature fruit. The microfissure/dehydration phenotype is inherited as a single gene, termed Cwp1 (cuticular water permeability). The gene was fine mapped, and its identity was determined by map-based cloning and differential expression analysis in near-isogenic lines. Causality of the Cwp1 gene was shown by the heterologous transgenic expression of the gene in the cultivated tomato, which caused a microfissured fruit cuticle leading to dehydrated fruit. Cwp1 encodes for a protein of unidentified function in the DUF833 domain family. The gene is expressed in the fruit epidermis of the dehydrating genotype harbouring the wild-species introgression, but not in the cultivated tomato. It is expressed only in the primitive green-fruited wild tomato species, but is not expressed in the cultivated Solanum lycopersicum and the closely related Solanum cheesmaniae and Solanum pimpinellifolium, indicating a pre-adaptive role for Cwp1 silencing in the evolution and domestication of the cultivated tomato. © 2007 The Authors.
Scientific Publication
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