אסיף מאגר המחקר החקלאי

Particle bombardment-mediated transformation of ornithogalum dubium for ornithogalum mosaic virus resistance

Year:

2005

Source of publication :

Authors :

Volume :

673

Co-Authors:

Cohen, A., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Lipsky, A., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Arazi, T., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Ion, A., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Stav, R., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Sandler-Ziv, D., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Fintea, C., Dept. Ornamental Horticulture, ARO, Volcani Center, Bet Dagan 50250, Israel
Gaba, V., Dept. Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gera, A., Dept. Virology, ARO, Volcani Center, Bet Dagan 50250, Israel

Facilitators :

Link :

Abstract:

Ornithogalum mosaic virus (OrMV) causes flower deformation and deterioration of planting stocks in species and hybrids of Ornithogalum and Lachenalia. No resistance to viral infection by OrMV is known, making utilization of transformation technologies the natural choice for the introduction of virus resistance. Transformation with viral coat protein (CP) and replicase genes has been shown to confer resistance to viral infection in many plant species. Liquid-grown cell clusters of O. dubium were bombarded with gold particles coated with a plasmid carrying nptII gene, conferring kanamycin resistance, GUS reporter gene, and either CP gene or the viral replicase (NIb) target genes under the control of either polyubiquitin (UBQ3) or the strawberry vein-banding virus deleted (ΔSVB) promoters. Following prolonged selection in a liquid medium supplemented with 80 mg/l kanamycin in darkness, the cultures were transferred to regeneration medium in the light, where hundreds of putative transgenic plantlets developed. Most of the regenerated plants were GUS-positive. PCR analysis indicated the presence of GUS reporter gene and nptII selectable gene, and either the CP or replicase transgenes. Transgenic plants are being propagated vegetatively before being challenged with virus infection to confirm their state of resistance.

Note:

Related Files :

תוכן קשור

More details

You may also be interested in