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פותח על ידי קלירמאש פתרונות בע"מ -
Local production of the gonadotropic hormones in the rat ovary
Year:
2008
Authors :
גרשון, ערן
;
.
Volume :
282
Co-Authors:
Schirman-Hildesheim, T.D., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Gershon, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Litichever, N., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Galiani, D., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Ben-Aroya, N., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Dekel, N., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Koch, Y., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
32
To page:
38
(
Total pages:
7
)
Abstract:
The gonadotropic hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are synthesized by and released from the anterior pituitary in response to the hypothalamic gonadotropin-releasing hormone (GnRH) signaling. In the female, LH and FSH affect folliculogenesis, ovarian steroid production, oocyte maturation, ovulation and corpus luteum formation. We have recently studied the expression of GnRH and its receptor in the rat ovary and found organ-specific, estrous cycle-dependant, fluctuations. Subsequently, we wished to determine whether rat ovaries also express gonadotropic hormones. Using RT-PCR, we detected LHβ, FSHβ and the common α-subunit mRNA's in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. Granulosa cells, however, express mRNA's for LHβ and the common α-subunit, but not for FSHβ. We cloned and sequenced the ovarian LHβ transcript and found it to be longer (2.3 kb) than the one produced by pituitary gonadotropes (0.8 kb), due to a longer 5′-UTR. We studied the regulation of ovarian LHβ mRNA in sexually immature female rats administered with pregnant mare serum gonadotropin (PMSG) and in adult cyclic rats. PMSG administration caused a significant decrease in LHβ mRNA expression, detected by real-time PCR. Similarly, LHβ mRNA levels were lower on estrous morning versus proestrous evening. Interestingly, ovarian content of LH remained unchanged following hypophysectomy, although ovarian weight was immensely reduced. Taken together, it seems probable that ovarian LH is heterologously/homologously regulated by pituitary, and possibly also by local gonadotropins. Thus, these findings may imply the existence of a local GnRH-gonadotropin axis in the mammalian ovary that may be involved in the management of processes that lead to ovulation. © 2007 Elsevier Ireland Ltd. All rights reserved.
Note:
Related Files :
animal experiment
Animals
animal tissue
Female
Granulosa Cells
hormone synthesis
ovulation
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.mce.2007.11.014
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20705
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
You may also be interested in
Scientific Publication
Local production of the gonadotropic hormones in the rat ovary
282
Schirman-Hildesheim, T.D., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Gershon, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Litichever, N., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Galiani, D., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Ben-Aroya, N., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Dekel, N., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel
Koch, Y., Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel
Local production of the gonadotropic hormones in the rat ovary
The gonadotropic hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are synthesized by and released from the anterior pituitary in response to the hypothalamic gonadotropin-releasing hormone (GnRH) signaling. In the female, LH and FSH affect folliculogenesis, ovarian steroid production, oocyte maturation, ovulation and corpus luteum formation. We have recently studied the expression of GnRH and its receptor in the rat ovary and found organ-specific, estrous cycle-dependant, fluctuations. Subsequently, we wished to determine whether rat ovaries also express gonadotropic hormones. Using RT-PCR, we detected LHβ, FSHβ and the common α-subunit mRNA's in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. Granulosa cells, however, express mRNA's for LHβ and the common α-subunit, but not for FSHβ. We cloned and sequenced the ovarian LHβ transcript and found it to be longer (2.3 kb) than the one produced by pituitary gonadotropes (0.8 kb), due to a longer 5′-UTR. We studied the regulation of ovarian LHβ mRNA in sexually immature female rats administered with pregnant mare serum gonadotropin (PMSG) and in adult cyclic rats. PMSG administration caused a significant decrease in LHβ mRNA expression, detected by real-time PCR. Similarly, LHβ mRNA levels were lower on estrous morning versus proestrous evening. Interestingly, ovarian content of LH remained unchanged following hypophysectomy, although ovarian weight was immensely reduced. Taken together, it seems probable that ovarian LH is heterologously/homologously regulated by pituitary, and possibly also by local gonadotropins. Thus, these findings may imply the existence of a local GnRH-gonadotropin axis in the mammalian ovary that may be involved in the management of processes that lead to ovulation. © 2007 Elsevier Ireland Ltd. All rights reserved.
Scientific Publication
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