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פותח על ידי קלירמאש פתרונות בע"מ -
Studies of the trehalase activity in eggs of the egyptian cotton worm, Spodoptera littoralis
Year:
1978
Source of publication :
Insect Biochemistry
Authors :
יבלונסקי, שרה
;
.
ישעיה, יצחק
;
.
Volume :
8
Co-Authors:
Ishaaya, I., Division of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Yablonski, S., Division of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
81
To page:
85
(
Total pages:
5
)
Abstract:
In eggs of Spodoptera littoralis, the optimum conditions for trehalase activity involve a reaction mixture of 0.05 M acetate buffer (pH 3.5) and 1.5% trehalose at 37°C for 60 min. A catalytic period of up to 90 min was found to be linear, the Km value was 0.03 M, and the enzyme activity reached its maximum at 55°C. In the presence of sodium deoxycholate, the trehalase activity was strongly enhanced, indicating the presence of an active system of membrane-bound trehalase. A decrease of about 20% in the activity of the membrane enzyme was observed in the first instar larvae shortly before hatching, with no appreciable change in the activity of the soluble enzyme. Urea and divalent cations were found to suppress considerably the enzyme activity, HgCl2 being the most effective compound. © 1978.
Note:
Related Files :
trehalase activity divalent cations effect on
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0020-1790(78)90014-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20709
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
You may also be interested in
Scientific Publication
Studies of the trehalase activity in eggs of the egyptian cotton worm, Spodoptera littoralis
8
Ishaaya, I., Division of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Yablonski, S., Division of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Studies of the trehalase activity in eggs of the egyptian cotton worm, Spodoptera littoralis
In eggs of Spodoptera littoralis, the optimum conditions for trehalase activity involve a reaction mixture of 0.05 M acetate buffer (pH 3.5) and 1.5% trehalose at 37°C for 60 min. A catalytic period of up to 90 min was found to be linear, the Km value was 0.03 M, and the enzyme activity reached its maximum at 55°C. In the presence of sodium deoxycholate, the trehalase activity was strongly enhanced, indicating the presence of an active system of membrane-bound trehalase. A decrease of about 20% in the activity of the membrane enzyme was observed in the first instar larvae shortly before hatching, with no appreciable change in the activity of the soluble enzyme. Urea and divalent cations were found to suppress considerably the enzyme activity, HgCl2 being the most effective compound. © 1978.
Scientific Publication
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