Co-Authors:
Yosef, E., Metabolic Unit, Institute of Animal Science, ARO, Bet Dagan, 50250, Israel
Ben-Ghedalia, D., Metabolic Unit, Institute of Animal Science, ARO, Bet Dagan, 50250, Israel
Miron, J., Metabolic Unit, Institute of Animal Science, ARO, Bet Dagan, 50250, Israel
Huttermann, A., Forstbotanisches Institut, Universität Göttingen, D-3400, Busgenweg 2, Göttingen, Germany
Majcherczyk, A., Forstbotanisches Institut, Universität Göttingen, D-3400, Busgenweg 2, Göttingen, Germany
Milstein, O., Forstbotanisches Institut, Universität Göttingen, D-3400, Busgenweg 2, Göttingen, Germany
Ludemann, H.D., Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, 8400 Regensburg, Germany
Frund, R., Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, 8400 Regensburg, Germany
Abstract:
Chemical and physical properties of cell walls (CW) and CW components have been studied in untreated wheat straw (WS) and SO2-treated wheat straw (SO2-WS). The concentration of water-soluble total free monomeric phenolics was 47.1 mg per 100 g and 129 mg per 100 g straw dry matter in WS and SO2-WS, respectively. The major solubilizing effect of the SO2 treatment was exerted on ferulic acid, the concentration of which increased tenfold. Matrix complexes extracted by 1 N NaOH from CW of wheat straw subjected to ball-milling plus Trichoderma reesei cellulase pretreatment underwent high-per-formance size exclusion chromatography to assess the molecular weight distribution. Most (84%) of the matrix complexes extracted from WS were in the range of 1600-23000 Da, whereas those originating from SO2-WS showed a more dispersed pattern, with molecular weights of up to and beyond 125 000 Da. The weight average molecular weight (Mwt) was 10 500 Da and 35 000 Da for the matrix complexes of WS and SO2-WS, respectively. Total monosaccharide content of matrix complexes was 36.4 g per 100 g and 42.5 g per 100 g in WS and SO2-WS, respectively, with xylose as the major (70-80%) component. Solid-state cross-polarization-magic angle spinning 13C NMR spectroscopy of neutral detergent fiber preparations of wheat straw showed a decline in the intensity of signals at 89 ppm and 65 ppm in response to SO2 treatment and in vitro digestion, respectively. The distribution of carbons in CW chemical groups and components as determined by the NMR analysis was found to be close to values obtained by wet chemistry. © 1994.
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