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פותח על ידי קלירמאש פתרונות בע"מ -
Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
Year:
2014
Source of publication :
Clinical Cancer Research
Authors :
סרוסי, אייל
;
.
פרידמן-עינת, מרים
;
.
רוזל, מרק
;
.
שינדר, דמיטרי
;
.
Volume :
217
Co-Authors:
Gertler, A., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shinder, D., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Yosefi, S., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Shpilman, M., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Rosenblum, C.I., Merck and Company Inc., Whitehouse Station (C.I.R.), NJ 07065, United States
Ruzal, M., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Seroussi, E., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Freidman-Einat, M., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Gertler, A., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shpilman, M., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Facilitators :
From page:
180
To page:
184
(
Total pages:
5
)
Abstract:
A chicken gene orthologous to human leptin receptor (LEPR) has been characterized and found to be active in leptin signaling in vitro in response to a variety of recombinant leptins and leptin-containing blood samples. However, the endogenous ligand of chicken LEPR(cLEPR) -the putative chicken leptin -has been reported by us and others to be undetectable at the DNA, mRNA, protein and activity levels. These reports have raised questions as to cLEPR's role. Here we analyzed the effects of a pegylated superactive mouse leptin antagonist (PEG-SMLA) in chicken. We showed that the leptin antagonist efficiently and specifically blocks leptin signaling through the cLEPR in vitro. The effect of the leptin antagonist was then studied in vivo by daily administration of 10 mg kg-1 for 10 consecutive days to white leghorn female chickens (Gallus gallus) at the age of 2 weeks. Despites the efficient attenuation of the cLEPRin vitro, no effect was observed on body mass, feed intake, feed efficiency or fat accumulation in the treated birds. Because similar treatment in rodents leads to a highly pronounced increase in appetite and body mass that are observed from the first day of treatment, it is concluded that the cLEPR is not implicated in the control of appetite or adipose homeostasis in chickens. © 2014. Published by The Company of Biologists Ltd.
Note:
Related Files :
Animals
Chicken leptin
Chickens
eating
Female
Leptin antagonist
Male
mice
Receptors, Leptin
signal transduction
עוד תגיות
תוכן קשור
More details
DOI :
10.1242/jeb.095539
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20727
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
Scientific Publication
Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
217
Gertler, A., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shinder, D., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Yosefi, S., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Shpilman, M., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Rosenblum, C.I., Merck and Company Inc., Whitehouse Station (C.I.R.), NJ 07065, United States
Ruzal, M., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Seroussi, E., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Freidman-Einat, M., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Gertler, A., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shpilman, M., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
A chicken gene orthologous to human leptin receptor (LEPR) has been characterized and found to be active in leptin signaling in vitro in response to a variety of recombinant leptins and leptin-containing blood samples. However, the endogenous ligand of chicken LEPR(cLEPR) -the putative chicken leptin -has been reported by us and others to be undetectable at the DNA, mRNA, protein and activity levels. These reports have raised questions as to cLEPR's role. Here we analyzed the effects of a pegylated superactive mouse leptin antagonist (PEG-SMLA) in chicken. We showed that the leptin antagonist efficiently and specifically blocks leptin signaling through the cLEPR in vitro. The effect of the leptin antagonist was then studied in vivo by daily administration of 10 mg kg-1 for 10 consecutive days to white leghorn female chickens (Gallus gallus) at the age of 2 weeks. Despites the efficient attenuation of the cLEPRin vitro, no effect was observed on body mass, feed intake, feed efficiency or fat accumulation in the treated birds. Because similar treatment in rodents leads to a highly pronounced increase in appetite and body mass that are observed from the first day of treatment, it is concluded that the cLEPR is not implicated in the control of appetite or adipose homeostasis in chickens. © 2014. Published by The Company of Biologists Ltd.
Scientific Publication
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