אסיף מאגר המחקר החקלאי

Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs

Year:

2016

Source of publication :

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Volume :

99

Co-Authors:

Hood-Nowotny, R., Austrian Institute of Technology, Vienna, Austria, Department of Chemical Ecology and Ecosystem Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, Vienna, Austria, AIT, Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, Tulln, Austria
Harari, A., Ministry of Agriculture and Rural Development, Agricultural Research Organization Volcani Centre, Department of Entomology, P.O. Box 6, Bet Dagan, Israel
Seth, R.K., Department of Zoology, University of Delhi, New Delhi, India
Wee, S.L., Universiti Kebangsaan Malaysia, School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Bangi, Selangor Darul Ehsan, Malaysia
Conlong, D.E., South African Sugarcane Research Institute (SASRI), 170 Flanders Drive, Mount Edgecombe, South Africa, Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X01, Matieland, South Africa
Suckling, D.M., Horticultural and Food Institute, New Zealand Institute for Plant and Food Research Ltd, Mt Albert Research Centre, 120 Mt Albert Road, Private Bag 92169, Auckland, Canterbury, New Zealand
Woods, B., Department of Agriculture and Food, 3 Baron Hay Ct., South Perth, WA, Australia
Lebdi-Grissa, K., Institut National Agronomique de Tunisie, 43, avenue Charles Nicole, Tunisia, Tunisia
Simmons, G., USDA-APHIS-PPQ-CPHST, 1636 E. Alisal, Salinas, CA, United States
Carpenter, J.E., USDA-ARS-CPMRU, 2747 Davis Road, Tifton, GA, United States

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Abstract:

In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around -27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around -11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner. © International Atomic Energy Agency 2016. Published by the Florida Entomological Society. All rights reserved.

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