אסיף מאגר המחקר החקלאי

Spirodela (duckweed) as an alternative production system for pharmaceuticals: A case study, aprotinin

Year:

2008

Source of publication :

Authors :

Volume :

17

Co-Authors:

Rival, S., LemnaGene SA, 71 Chemin du Moulin Carron, Dardilly 69570, France
Wisniewski, J.-P., LemnaGene SA, 71 Chemin du Moulin Carron, Dardilly 69570, France
Langlais, A., LemnaGene SA, 71 Chemin du Moulin Carron, Dardilly 69570, France
Kaplan, H., LemnaGene SA, 71 Chemin du Moulin Carron, Dardilly 69570, France
Freyssinet, G., LemnaGene SA, 71 Chemin du Moulin Carron, Dardilly 69570, France, Limagrain, BPNO. 1, Chappes 63720, France
Vancanneyt, G., Bayer BioScience NV, Technologiepark 38, Gent B-9052, Belgium
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Perl, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Bet-Dagan, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel

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Abstract:

Aprotinin is a small serine protease inhibitor used in human health. Spirodela were transformed, via Agrobacterium, with a synthetic gene encoding the mature aprotinin sequence and a signal peptide for secretion which was driven by the CaMV 35S promoter. A total of 25 transgenic Spirodela lines were generated and aprotinin production was confirmed by northern and western blot analyses. Expression levels of up to 3.7% of water soluble proteins were detected in the plant and 0.65 mg/l in the growth medium. In addition, immunoaffinity purification of the protein followed by amino acid sequencing confirmed the correct splicing of the aprotinin produced in Spirodela and secreted into the growth medium. © 2007 Springer Science+Business Media B.V.

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