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חיפוש מתקדם
Israel Journal of Plant Sciences
Levin, I., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cincarevsky, F., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Oguz, I., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Petreikov, M., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yeselson, Y., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shen, S., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar, M., Gedera Seed Company, P.O. Box 103, Gedera 70750, Israel
Schaffer, A.A., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
A DNA locus, FGR, highly associated with increased fructose-to-glucose ratio in mature tomato fruits, was previously identified and mapped to the centromeric region of the tomato chromosome 4. In order to identify the genes that modulate fructose-to-glucose ratio, a battery of candidate genes coding for key enzymes in the carbohydrate metabolic pathway were mapped, and their polymorphism between Lycopersicon hirsutum-derived fructose accumulation lines and cultivated L. esculentum lines detected. Our results show that the L. hirsutum allelic form of fructokinase 2 gene (FK2) is present, in conjunction with the FGR allele, also derived from L. hirsutum, in breeding lines characterized by higher fructose-to-glucose levels (>2.0). Analysis of F 2 and F3 populations segregating for both FGR and FK2 shows that the FK2 locus is associated with fructose-to-glucose ratio in the fruit in an epistatic manner: in the presence of the L. hirsutum FGR allele, the L. hirsutum FK2 allele further increases fructose-to-glucose ratio in a more than additive manner. Analysis of the HEXOKINASE 2 gene (HK2), mapped 6 cM north of FK2 on the tomato chromosome 6 had, on the other hand, no effect on fructose-to-glucose ratio. FK2 can thus serve as an additional DNA marker to further increase fructose-to-glucose ratio. © 2006 Science From Israel/LPPLtd.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Epistatic interaction between two unlinked loci derived from introgressions from Lycopersicon hirsutum further modulates the fructose-to-glucose ratio in the mature tomato fruit
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Levin, I., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cincarevsky, F., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Oguz, I., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Petreikov, M., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yeselson, Y., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shen, S., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar, M., Gedera Seed Company, P.O. Box 103, Gedera 70750, Israel
Schaffer, A.A., Department of Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Epistatic interaction between two unlinked loci derived from introgressions from Lycopersicon hirsutum further modulates the fructose-to-glucose ratio in the mature tomato fruit
A DNA locus, FGR, highly associated with increased fructose-to-glucose ratio in mature tomato fruits, was previously identified and mapped to the centromeric region of the tomato chromosome 4. In order to identify the genes that modulate fructose-to-glucose ratio, a battery of candidate genes coding for key enzymes in the carbohydrate metabolic pathway were mapped, and their polymorphism between Lycopersicon hirsutum-derived fructose accumulation lines and cultivated L. esculentum lines detected. Our results show that the L. hirsutum allelic form of fructokinase 2 gene (FK2) is present, in conjunction with the FGR allele, also derived from L. hirsutum, in breeding lines characterized by higher fructose-to-glucose levels (>2.0). Analysis of F 2 and F3 populations segregating for both FGR and FK2 shows that the FK2 locus is associated with fructose-to-glucose ratio in the fruit in an epistatic manner: in the presence of the L. hirsutum FGR allele, the L. hirsutum FK2 allele further increases fructose-to-glucose ratio in a more than additive manner. Analysis of the HEXOKINASE 2 gene (HK2), mapped 6 cM north of FK2 on the tomato chromosome 6 had, on the other hand, no effect on fructose-to-glucose ratio. FK2 can thus serve as an additional DNA marker to further increase fructose-to-glucose ratio. © 2006 Science From Israel/LPPLtd.
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