Co-Authors:
HADAS, R., S. Tolkowsky Laboratory, Department of Virology, ARO, Volcani Center, Bet Dagan, Israel
BAR‐JOSEPH, M., S. Tolkowsky Laboratory, Department of Virology, ARO, Volcani Center, Bet Dagan, Israel
SEMANCIK, J.S., Department of Plant Pathology, University of California Riverside, California, United States
Abstract:
A marked variation in tree size occurred in grapefruit trees budded in trifoliate orange rootstock and inoculated with a graft‐transmissible dwarfing agent (GTD) from grapefruit. Etrog citron (Citrus medico) inoculated with budwood collected from two GTD sub‐types displaying very mild (VM) and mild (M) dwarfing effects showed only mild leaf epinasty, whereas the severe (S) subtype induced severe epinasty and stunting typical of infection by the original GTD source 225‐T. The characteristic symptoms of the three sub‐types were persistent following three serial transfers to ‘Etrog’ indicators. Extracts from ‘Etrog’ citron containing either the 225‐T or its derivative S displayed a profile of five viroid bands when analysed by the sequential PAGE system for detection of circular RNAs. The profile obtained included the well characterised 371 nucleotide citrus exocortis viroid (CEV) and four additional viroids of approximately 330, 300, 295 and 275 nucleotides. Sub‐types M and VM lacked the CEV band and each contained a complement of only three viroids of 330, 295, 275 and 300, 295, 275 nucleotides, respectively. These results indicate that a segregation of the viroid complex in grapefruit budwood was a major factor in the variation seen among trees inoculated from the GTD source 225‐T. Copyright © 1989, Wiley Blackwell. All rights reserved
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