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פותח על ידי קלירמאש פתרונות בע"מ -
Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures
Year:
1993
Authors :
מנדלבאום, רפי
;
.
Volume :
59
Co-Authors:
Mandelbaum, R.T., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Wackett, L.P., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Allan, D.L., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Facilitators :
From page:
1695
To page:
1701
(
Total pages:
7
)
Abstract:
Enrichment cultures containing atrazine (2-chloro-4-ethylamino-6- isopropylamino-1,3,5-triazine) at a concentration of 100 ppm (0.46 mM) as a sole nitrogen source were obtained from soils exposed to repeated spills of atrazine, alachlor, and metolachlor. Bacterial growth occurred concomitantly with formation of metabolites from atrazine and subsequent biosynthesis of protein. When ring-labeled [14C]atrazine was used, 80% or more of the s- triazine ring carbon atoms were liberated as 14CO2. Hydroxyatrazine may be an intermediate in the atrazine mineralization pathway. More than 200 pure cultures isolated from the enrichment cultures failed to utilize atrazine as a nitrogen source. Mixing pure cultures restored atrazine-mineralizing activity. Repeated transfer of the mixed cultures led to increased rates of atrazine metabolism. The rate of atrazine degradation, even at the elevated concentrations used, far exceeded the rates previously reported in soils, waters, and mixed and pure cultures of bacteria.
Note:
Related Files :
Atrazine
bacteria
bacterium culture
Biodegradation
nitrogen
Sampling
soil
water
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20836
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:39
Scientific Publication
Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures
59
Mandelbaum, R.T., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Wackett, L.P., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Allan, D.L., Department of Biochemistry, IASBPT, University of Minnesota, St. Paul, MN 55108, United States
Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures
Enrichment cultures containing atrazine (2-chloro-4-ethylamino-6- isopropylamino-1,3,5-triazine) at a concentration of 100 ppm (0.46 mM) as a sole nitrogen source were obtained from soils exposed to repeated spills of atrazine, alachlor, and metolachlor. Bacterial growth occurred concomitantly with formation of metabolites from atrazine and subsequent biosynthesis of protein. When ring-labeled [14C]atrazine was used, 80% or more of the s- triazine ring carbon atoms were liberated as 14CO2. Hydroxyatrazine may be an intermediate in the atrazine mineralization pathway. More than 200 pure cultures isolated from the enrichment cultures failed to utilize atrazine as a nitrogen source. Mixing pure cultures restored atrazine-mineralizing activity. Repeated transfer of the mixed cultures led to increased rates of atrazine metabolism. The rate of atrazine degradation, even at the elevated concentrations used, far exceeded the rates previously reported in soils, waters, and mixed and pure cultures of bacteria.
Scientific Publication
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