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פותח על ידי קלירמאש פתרונות בע"מ -
Ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro
Year:
2007
Source of publication :
Plant Cell Reports
Authors :
גאבה, ויקטור
;
.
זינגר, סימה
;
.
יבלונסקי, שרה
;
.
מורוגננטם, מוקאן
;
.
פישר, ארווין
;
.
שיה, שאודי
;
.
Volume :
26
Co-Authors:
Ananthakrishnan, G., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850-2299, United States
Xia, X., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Amutha, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Singer, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Muruganantham, M., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yablonsky, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Fischer, E., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, SEM Unit, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
267
To page:
276
(
Total pages:
10
)
Abstract:
Ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (Cucurbita pepo L.) cultivars Ma'yan and Bareqet, on Murashige and Skoog [Physiol Plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 μM benzyladenine. At this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. Ultrasound also stimulated massive explant growth. Ultrasound treatment resulted in further multiple shoot production (five times greater than control) after explant transfer to elongation medium (Murashige and Skoog [Physiol Plant 15:473-497, 1962] medium with 0.44 μM benzyladenine and 2.9 μM gibberellic acid). Longer ultrasonic treatments (5 or 10 min) promoted multiple shoot regeneration and explant growth accompanied by hyperhydration. Scanning electron microscope observations showed that 2 min ultrasound changed the joint area between epidermal cells and removed some of the surface from the cotyledon epidermal cells, without gross surface injury to the explants. Longer periods of ultrasound (5-10 min) caused further surface erosion. Rubbing the explant contact surface with chloroform or sandpaper emulated the effect of sonication on shoot regeneration and explant growth, demonstrating that ultrasound exerts its morphogenic influence by surface removal. Sonication of explants from other batches of squash seeds (of cultivars Ma'yan and True French), that regenerated without such treatment, reduced regeneration and caused hyperhydration. This is the first report of stimulation of in vitro regeneration by ultrasound treatment. © 2006 Springer-Verlag.
Note:
Related Files :
Cotyledon
Cucurbita
Cucurbita pepo
Growth
plant
plant growth
Plants
ultrasonics
ultrastructure
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00299-006-0235-1
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20856
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:39
Scientific Publication
Ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro
26
Ananthakrishnan, G., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850-2299, United States
Xia, X., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Amutha, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Singer, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Muruganantham, M., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yablonsky, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Fischer, E., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, SEM Unit, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, ARO Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro
Ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (Cucurbita pepo L.) cultivars Ma'yan and Bareqet, on Murashige and Skoog [Physiol Plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 μM benzyladenine. At this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. Ultrasound also stimulated massive explant growth. Ultrasound treatment resulted in further multiple shoot production (five times greater than control) after explant transfer to elongation medium (Murashige and Skoog [Physiol Plant 15:473-497, 1962] medium with 0.44 μM benzyladenine and 2.9 μM gibberellic acid). Longer ultrasonic treatments (5 or 10 min) promoted multiple shoot regeneration and explant growth accompanied by hyperhydration. Scanning electron microscope observations showed that 2 min ultrasound changed the joint area between epidermal cells and removed some of the surface from the cotyledon epidermal cells, without gross surface injury to the explants. Longer periods of ultrasound (5-10 min) caused further surface erosion. Rubbing the explant contact surface with chloroform or sandpaper emulated the effect of sonication on shoot regeneration and explant growth, demonstrating that ultrasound exerts its morphogenic influence by surface removal. Sonication of explants from other batches of squash seeds (of cultivars Ma'yan and True French), that regenerated without such treatment, reduced regeneration and caused hyperhydration. This is the first report of stimulation of in vitro regeneration by ultrasound treatment. © 2006 Springer-Verlag.
Scientific Publication
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