נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Development of an enzyme-linked immunosorbent assay and a beta-1 adrenergic receptor-based assay for monitoring the drug atenolol
Year:
2013
Authors :
אלטשטיין, מרים
;
.
ברונשטיין, אליסה
;
.
חריטון, עליזה
;
.
ספיר, א'
;
.
סקלקה, ניר
;
.
Volume :
32
Co-Authors:

Sapir, A., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Shalev, A. Hariton, Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Skalka, N., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Bronshtein, A., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Altstein, M., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel

Facilitators :
From page:
585
To page:
593
(
Total pages:
9
)
Abstract:
Two approaches for monitoring atenolol (ATL) were applied: an immunochemical assay and a competitive-binding assay, based on the interaction between ATL and its target receptor, β1 adrenergic receptor (β1AR). Polyclonal antibodies (Abs) for ATL were generated, and a highly specific microplate immunochemical assay, that is, an enzyme-linked immunosorbent assay (ELISA), for its detection was developed. The ATL ELISA exhibited I50 and limit of detection (I20) values of 0.15±0.048 and 0.032±0.016ng/ml, respectively, and the Abs did not cross-react with any of the tested beta-blocker drugs. Furthermore, a human β1AR (h-β1AR) was stably expressed in Spodoptera frugiperda cells (Sf9). The receptor was employed to develop a competitive-binding assay that monitored binding of ATL in the presence of isoproteranol by quantification of secondary messenger, cyclic adenosine monophosphate (cAMP), levels in the transfected cells. The assay showed that the recombinant h-β1AR was functional, could bind the agonistic ligand isoproterenol as well as the antagonist ATL, as indicated by a dose-dependent elevation of cAMP in the presence of isoproteranol, and decrease after ATL addition. The highly efficient and sensitive ELISA and the receptor assay represent two methods suitable for efficient and cost-effective large-scale, high-throughput monitoring of ATL in environmental, agricultural, and biological samples. Environ. Toxicol. Chem. 2013;32:585-593. © 2012 SETAC.
Note:
Related Files :
animal cell
betaxolol
Bioassay
chemistry
cost effectiveness analysis
Cyclic AMP
environmental monitoring
propranolol
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/etc.2078
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20861
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:39
Scientific Publication
Development of an enzyme-linked immunosorbent assay and a beta-1 adrenergic receptor-based assay for monitoring the drug atenolol
32

Sapir, A., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Shalev, A. Hariton, Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Skalka, N., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Bronshtein, A., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel
Altstein, M., Department of Entomology, Institute of Plant Protection, Volcani Center, Bet Dagan, Israel

Development of an enzyme-linked immunosorbent assay and a beta-1 adrenergic receptor-based assay for monitoring the drug atenolol
Two approaches for monitoring atenolol (ATL) were applied: an immunochemical assay and a competitive-binding assay, based on the interaction between ATL and its target receptor, β1 adrenergic receptor (β1AR). Polyclonal antibodies (Abs) for ATL were generated, and a highly specific microplate immunochemical assay, that is, an enzyme-linked immunosorbent assay (ELISA), for its detection was developed. The ATL ELISA exhibited I50 and limit of detection (I20) values of 0.15±0.048 and 0.032±0.016ng/ml, respectively, and the Abs did not cross-react with any of the tested beta-blocker drugs. Furthermore, a human β1AR (h-β1AR) was stably expressed in Spodoptera frugiperda cells (Sf9). The receptor was employed to develop a competitive-binding assay that monitored binding of ATL in the presence of isoproteranol by quantification of secondary messenger, cyclic adenosine monophosphate (cAMP), levels in the transfected cells. The assay showed that the recombinant h-β1AR was functional, could bind the agonistic ligand isoproterenol as well as the antagonist ATL, as indicated by a dose-dependent elevation of cAMP in the presence of isoproteranol, and decrease after ATL addition. The highly efficient and sensitive ELISA and the receptor assay represent two methods suitable for efficient and cost-effective large-scale, high-throughput monitoring of ATL in environmental, agricultural, and biological samples. Environ. Toxicol. Chem. 2013;32:585-593. © 2012 SETAC.
Scientific Publication
You may also be interested in