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פותח על ידי קלירמאש פתרונות בע"מ -
Transgenic expression of the rice Xa21 pattern-recognition receptor in banana (Musa sp.) confers resistance to Xanthomonas campestris pv. musacearum
Year:
2014
Source of publication :
Plant Biotechnology Journal
Authors :
בהר, אופיר
;
.
Volume :
12
Co-Authors:
Tripathi, J.N., International Institute of Tropical Agriculture (IITA), Nairobi, Kenya
Lorenzen, J., International Institute of Tropical Agriculture (IITA), Arusha, Tanzania
Bahar, O., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Ronald, P., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Tripathi, L., International Institute of Tropical Agriculture (IITA), Nairobi, Kenya
Facilitators :
From page:
663
To page:
673
(
Total pages:
11
)
Abstract:
Banana Xanthomonas wilt (BXW), caused by the bacterium Xanthomonas campestris pv. musacearum (Xcm), is the most devastating disease of banana in east and central Africa. The spread of BXW threatens the livelihood of millions of African farmers who depend on banana for food security and income. There are no commercial chemicals, biocontrol agents or resistant cultivars available to control BXW. Here, we take advantage of the robust resistance conferred by the rice pattern-recognition receptor (PRR), XA21, to the rice pathogen Xanthomonas oryzae pv. oryzae (Xoo). We identified a set of genes required for activation of Xa21-mediated immunity (rax) that were conserved in both Xoo and Xcm. Based on the conservation, we hypothesized that intergeneric transfer of Xa21 would confer resistance to Xcm. We evaluated 25 transgenic lines of the banana cultivar 'Gonja manjaya' (AAB) using a rapid bioassay and 12 transgenic lines in the glasshouse for resistance against Xcm. About 50% of the transgenic lines showed complete resistance to Xcm in both assays. In contrast, all of the nontransgenic control plants showed severe symptoms that progressed to complete wilting. These results indicate that the constitutive expression of the rice Xa21 gene in banana results in enhanced resistance against Xcm. Furthermore, this work demonstrates the feasibility of PRR gene transfer between monocotyledonous species and provides a valuable new tool for controlling the BXW pandemic of banana, a staple food for 100 million people in east Africa. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
Note:
Related Files :
disease resistance
Genetics
Growth, Development and Aging
metabolism
Microbiology
Musa
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/pbi.12170
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20930
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
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Scientific Publication
Transgenic expression of the rice Xa21 pattern-recognition receptor in banana (Musa sp.) confers resistance to Xanthomonas campestris pv. musacearum
12
Tripathi, J.N., International Institute of Tropical Agriculture (IITA), Nairobi, Kenya
Lorenzen, J., International Institute of Tropical Agriculture (IITA), Arusha, Tanzania
Bahar, O., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Ronald, P., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Tripathi, L., International Institute of Tropical Agriculture (IITA), Nairobi, Kenya
Transgenic expression of the rice Xa21 pattern-recognition receptor in banana (Musa sp.) confers resistance to Xanthomonas campestris pv. musacearum
Banana Xanthomonas wilt (BXW), caused by the bacterium Xanthomonas campestris pv. musacearum (Xcm), is the most devastating disease of banana in east and central Africa. The spread of BXW threatens the livelihood of millions of African farmers who depend on banana for food security and income. There are no commercial chemicals, biocontrol agents or resistant cultivars available to control BXW. Here, we take advantage of the robust resistance conferred by the rice pattern-recognition receptor (PRR), XA21, to the rice pathogen Xanthomonas oryzae pv. oryzae (Xoo). We identified a set of genes required for activation of Xa21-mediated immunity (rax) that were conserved in both Xoo and Xcm. Based on the conservation, we hypothesized that intergeneric transfer of Xa21 would confer resistance to Xcm. We evaluated 25 transgenic lines of the banana cultivar 'Gonja manjaya' (AAB) using a rapid bioassay and 12 transgenic lines in the glasshouse for resistance against Xcm. About 50% of the transgenic lines showed complete resistance to Xcm in both assays. In contrast, all of the nontransgenic control plants showed severe symptoms that progressed to complete wilting. These results indicate that the constitutive expression of the rice Xa21 gene in banana results in enhanced resistance against Xcm. Furthermore, this work demonstrates the feasibility of PRR gene transfer between monocotyledonous species and provides a valuable new tool for controlling the BXW pandemic of banana, a staple food for 100 million people in east Africa. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
Scientific Publication
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