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The small subunit of snapdragon geranyl diphosphate synthase modifies the chain length specificity of tobacco geranylgeranyl diphosphate synthase in planta
Year:
2009
Source of publication :
Plant Cell
Authors :
פרידמן, איל
;
.
Volume :
21
Co-Authors:
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Nagegowda, D.A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States, Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Kish, C.M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Gutensohn, M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Maeda, H., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Varbanova, M., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Fridman, E., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Yamaguchi, S., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Hanada, A., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Kamiya, Y., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Krichevsky, A., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Pichersky, E., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Facilitators :
From page:
4002
To page:
4017
(
Total pages:
16
)
Abstract:
Geranyl diphosphate (GPP), the precursor of many monoterpene end products, is synthesized in plastids by a condensation of dimethylallyl diphosphate and isopentenyl diphosphate (IPP) in a reaction catalyzed by homodimeric or heterodimeric GPP synthase (GPPS). In the heterodimeric enzymes, a noncatalytic small subunit (GPPS.SSU) determines the product specificity of the catalytic large subunit, which may be either an active geranylgeranyl diphosphate synthase (GGPPS) or an inactive GGPPS-like protein. Here, we show that expression of snapdragon (Antirrhinum majus) GPPS.SSU in tobacco (Nicotiana tabacum) plants increased the total GPPS activity and monoterpene emission from leaves and flowers, indicating that the introduced catalytically inactive GPPS.SSU found endogenous large subunit partner(s) and formed an active snapdragon/tobacco GPPS in planta. Bimolecular fluorescence complementation and in vitro enzyme analysis of individual and hybrid proteins revealed that two of four GGPPS-like candidates from tobacco EST databases encode bona fide GGPPS that can interact with snapdragon GPPS.SSU and form a functional GPPS enzyme in plastids. The formation of chimeric GPPS in transgenic plants also resulted in leaf chlorosis, increased light sensitivity, and dwarfism due to decreased levels of chlorophylls, carotenoids, and gibberellins. In addition, these transgenic plants had reduced levels of sesquiterpene emission, suggesting that the export of isoprenoid intermediates from the plastids into the cytosol was decreased. These results provide genetic evidence that GPPS.SSU modifies the chain length specificity of phylogenetically distant GGPPS and can modulate IPP flux distribution between GPP and GGPP synthesis in planta. © 2009 American Society of Plant Biologists.
Note:
Related Files :
Antirrhinum
Antirrhinum majus
Diphosphates
Diterpenes
Genetics
metabolism
Monoterpenes
sesquiterpene
עוד תגיות
תוכן קשור
More details
DOI :
10.1105/tpc.109.071282
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20939
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
Scientific Publication
The small subunit of snapdragon geranyl diphosphate synthase modifies the chain length specificity of tobacco geranylgeranyl diphosphate synthase in planta
21
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Nagegowda, D.A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States, Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Kish, C.M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Gutensohn, M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Maeda, H., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Varbanova, M., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Fridman, E., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Yamaguchi, S., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Hanada, A., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Kamiya, Y., RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 2300045, Japan
Krichevsky, A., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Pichersky, E., Department of Molecular, Cellular, Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
The small subunit of snapdragon geranyl diphosphate synthase modifies the chain length specificity of tobacco geranylgeranyl diphosphate synthase in planta
Geranyl diphosphate (GPP), the precursor of many monoterpene end products, is synthesized in plastids by a condensation of dimethylallyl diphosphate and isopentenyl diphosphate (IPP) in a reaction catalyzed by homodimeric or heterodimeric GPP synthase (GPPS). In the heterodimeric enzymes, a noncatalytic small subunit (GPPS.SSU) determines the product specificity of the catalytic large subunit, which may be either an active geranylgeranyl diphosphate synthase (GGPPS) or an inactive GGPPS-like protein. Here, we show that expression of snapdragon (Antirrhinum majus) GPPS.SSU in tobacco (Nicotiana tabacum) plants increased the total GPPS activity and monoterpene emission from leaves and flowers, indicating that the introduced catalytically inactive GPPS.SSU found endogenous large subunit partner(s) and formed an active snapdragon/tobacco GPPS in planta. Bimolecular fluorescence complementation and in vitro enzyme analysis of individual and hybrid proteins revealed that two of four GGPPS-like candidates from tobacco EST databases encode bona fide GGPPS that can interact with snapdragon GPPS.SSU and form a functional GPPS enzyme in plastids. The formation of chimeric GPPS in transgenic plants also resulted in leaf chlorosis, increased light sensitivity, and dwarfism due to decreased levels of chlorophylls, carotenoids, and gibberellins. In addition, these transgenic plants had reduced levels of sesquiterpene emission, suggesting that the export of isoprenoid intermediates from the plastids into the cytosol was decreased. These results provide genetic evidence that GPPS.SSU modifies the chain length specificity of phylogenetically distant GGPPS and can modulate IPP flux distribution between GPP and GGPP synthesis in planta. © 2009 American Society of Plant Biologists.
Scientific Publication
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