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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
DNA barcoding of Israeli indigenous and introduced cichlids
Year:
2009
Authors :
בארוסו, רנאטה מלון
;
.
חולתא, גדעון
;
.
כהן-צינדר, מירי
;
.
סרוסי, אייל
;
.
רון, מיכה
;
.
שיראק, אנדרי
;
.
Volume :
61
Co-Authors:
Shirak, A., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Cohen-Zinder, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Barroso, R.M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
83
To page:
88
(
Total pages:
6
)
Abstract:
The objectives of this study were barcoding and taxonomic analysis of the five tilapiine species (Oreochromis aureus, O. niloticus, O. mossambicus, Sarotherodon galilaeus, and Tilapia zillii), two tilapia hybrid strains (Florida red tilapia and Philippine red tilapia), and two endemic wild cichlids (Tristramella simonis and Astatotilapia flaviijosephi) available in Israel,as well as O. urolepis hornorum. Cytochrome oxidase subunit I (COI) 619 bp sequence traces of 104 individuals were assembled, aligned, and compared (GenBank project GI 209553463). The DNA sequences of two hybrid strains were identical to those of O. hornorum and O. aureus. Absence of intra-specific variability was detected in the commercially used species, O. aureus, S. galilaeus, O. mossambicus, and O. urolepis hornorum. Two DNA sequence variants were detected in O. niloticus originating from Ghana and Egypt. In contrast, 2-3 variants were detected in the DNA of each of the non-commercial species. Amino-acid sequences were identical in all "true tilapias" and different from the sequences in the endemic cichlids. As a whole, the protein phylogenetic tree fitted the expected conventional taxonomy as opposed to the respective DNA-based tree. Sequences FJ348047-FJ348150 were submitted to GenBank via the BOLD database (identical to FISH001-08 - FISH104-08 in this database).
Note:
Related Files :
DNA
fish
Israel
Oreochromis aureus
Taxonomic analysis
Tilapia
Tristramella simonis
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20966
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
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Scientific Publication
DNA barcoding of Israeli indigenous and introduced cichlids
61
Shirak, A., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Cohen-Zinder, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Barroso, R.M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
DNA barcoding of Israeli indigenous and introduced cichlids
The objectives of this study were barcoding and taxonomic analysis of the five tilapiine species (Oreochromis aureus, O. niloticus, O. mossambicus, Sarotherodon galilaeus, and Tilapia zillii), two tilapia hybrid strains (Florida red tilapia and Philippine red tilapia), and two endemic wild cichlids (Tristramella simonis and Astatotilapia flaviijosephi) available in Israel,as well as O. urolepis hornorum. Cytochrome oxidase subunit I (COI) 619 bp sequence traces of 104 individuals were assembled, aligned, and compared (GenBank project GI 209553463). The DNA sequences of two hybrid strains were identical to those of O. hornorum and O. aureus. Absence of intra-specific variability was detected in the commercially used species, O. aureus, S. galilaeus, O. mossambicus, and O. urolepis hornorum. Two DNA sequence variants were detected in O. niloticus originating from Ghana and Egypt. In contrast, 2-3 variants were detected in the DNA of each of the non-commercial species. Amino-acid sequences were identical in all "true tilapias" and different from the sequences in the endemic cichlids. As a whole, the protein phylogenetic tree fitted the expected conventional taxonomy as opposed to the respective DNA-based tree. Sequences FJ348047-FJ348150 were submitted to GenBank via the BOLD database (identical to FISH001-08 - FISH104-08 in this database).
Scientific Publication
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