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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells
Year:
1998
Source of publication :
Plant Journal
Authors :
גפני, ידידיה
;
.
Volume :
13
Co-Authors:
Kunik, T., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Palanichelvam, K., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Czosnek, H., Department of Field, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Citovsky, V., Department of Biochemistry, State University of New York, Stony Brook, NY 11794-5215, United States
Gafni, Y., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
393
To page:
399
(
Total pages:
7
)
Abstract:
The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly-transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem-associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investigated by monitoring its specific nuclear accumulation in plant and insect cells. CP was fused to the β-glucuronidase (GUS) reporter enzyme to assay nuclear import in petunia protoplasts, and micro-injection of purified fluorescently labeled CP was used to examine its nuclear uptake in Drosophila embryos. Both assays demonstrated that TYLCV CP is transported into plant-and insect-cell nuclei by an active process of nuclear import via a nuclear localization signal (NLS)-specific pathway. Using the GUS assay and deletion analysis, the TYLCV CP NLS sequence was identified in the amino-terminus of the protein.
Note:
Related Files :
Animal
Animals
Genetics
metabolism
molecular genetics
Petunia
Plants
Tomato yellow leaf curl virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-313X.1998.00037.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20967
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
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Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells
13
Kunik, T., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Palanichelvam, K., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Czosnek, H., Department of Field, Otto Warburg Ctr. Biotech. in Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Citovsky, V., Department of Biochemistry, State University of New York, Stony Brook, NY 11794-5215, United States
Gafni, Y., Department of Genetics, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells
The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly-transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem-associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investigated by monitoring its specific nuclear accumulation in plant and insect cells. CP was fused to the β-glucuronidase (GUS) reporter enzyme to assay nuclear import in petunia protoplasts, and micro-injection of purified fluorescently labeled CP was used to examine its nuclear uptake in Drosophila embryos. Both assays demonstrated that TYLCV CP is transported into plant-and insect-cell nuclei by an active process of nuclear import via a nuclear localization signal (NLS)-specific pathway. Using the GUS assay and deletion analysis, the TYLCV CP NLS sequence was identified in the amino-terminus of the protein.
Scientific Publication
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