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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of a distinct carlavirus isolated from Verbena
Year:
2003
Source of publication :
Archives of Virology
Authors :
גרה, עבדאללה
;
.
מסלנין, לודמילה
;
.
פייגלסון, ליאוניד
;
.
רוזנר, אריה
;
.
Volume :
148
Co-Authors:
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Zeidan, M., Plant Protect./Inspection Services, Ministry of Agriculture, Bet Dagan, Israel
Feigelson, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1007
To page:
1015
(
Total pages:
9
)
Abstract:
A virus was isolated from Verbena plants that bore virus-like symptoms. The virus, for which the name Verbena latent virus (VeLV) is proposed, was consistently isolated from these plants, both with and without disease symptoms. Electron microscopy studies of ultrathin sections of infected Verbena tissues revealed the presence of elongated flexuous virus panicles, ca. 650 nm in length. Its experimental host range was limited to Verbena spp. and Nicotiana clevelandii. No inclusion bodies or specific cytopathological effects, were observed. Electrophoresis of dissociated purified virus preparation in sodium dodecyl sulfate-polyacrylamide gel revealed a major protein component with a molecular mass of 38.9 kDa. Polyclonal antibodies which could specifically bind to virus particles were produced. A portion of the viral RNA was cloned and sequenced; it comprised 2503 nucleotides and contained part of three open reading frames (ORFs) which from the 5′ to the 3′-ends, potentially encode for 489 amino acids (ORF1), a 25.8-kDa protein (ORF2) and a 12-kDa protein (ORF3). Comparison of the predicted amino acid sequence with those of other plant viruses revealed 40-60% identity with several carlaviruses. In the light of particle morphology, absence of specific cytopathological effects in ultrathin sections, and genomic and serological properties, it is suggested that this virus belongs to the genus Carlavirus.
Note:
Related Files :
Genes, Viral
Genome, Viral
Molecular Sequence Data
Open Reading Frames
Plant Leaves
Verbena
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00705-002-0973-5
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20984
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
You may also be interested in
Scientific Publication
Characterization of a distinct carlavirus isolated from Verbena
148
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Zeidan, M., Plant Protect./Inspection Services, Ministry of Agriculture, Bet Dagan, Israel
Feigelson, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Characterization of a distinct carlavirus isolated from Verbena
A virus was isolated from Verbena plants that bore virus-like symptoms. The virus, for which the name Verbena latent virus (VeLV) is proposed, was consistently isolated from these plants, both with and without disease symptoms. Electron microscopy studies of ultrathin sections of infected Verbena tissues revealed the presence of elongated flexuous virus panicles, ca. 650 nm in length. Its experimental host range was limited to Verbena spp. and Nicotiana clevelandii. No inclusion bodies or specific cytopathological effects, were observed. Electrophoresis of dissociated purified virus preparation in sodium dodecyl sulfate-polyacrylamide gel revealed a major protein component with a molecular mass of 38.9 kDa. Polyclonal antibodies which could specifically bind to virus particles were produced. A portion of the viral RNA was cloned and sequenced; it comprised 2503 nucleotides and contained part of three open reading frames (ORFs) which from the 5′ to the 3′-ends, potentially encode for 489 amino acids (ORF1), a 25.8-kDa protein (ORF2) and a 12-kDa protein (ORF3). Comparison of the predicted amino acid sequence with those of other plant viruses revealed 40-60% identity with several carlaviruses. In the light of particle morphology, absence of specific cytopathological effects in ultrathin sections, and genomic and serological properties, it is suggested that this virus belongs to the genus Carlavirus.
Scientific Publication
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