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פותח על ידי קלירמאש פתרונות בע"מ -
Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus
Year:
1987
Authors :
נאסר, אחמד
;
.
Volume :
53
Co-Authors:
Nasser, A.M., Department of Virology and Epidemiology, Baylor College of Medicine, Houston, TX 77030, United States
Metcalf, T.G., Department of Virology and Epidemiology, Baylor College of Medicine, Houston, TX 77030, United States
Facilitators :
From page:
2967
To page:
2971
(
Total pages:
5
)
Abstract:
Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm 3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.
Note:
Related Files :
Animal
animal cell
Centrifugation, Density Gradient
cytopathogenic effect
immunoglobulin G
radioimmunoassay
עוד תגיות
תוכן קשור
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DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21001
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
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Scientific Publication
Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus
53
Nasser, A.M., Department of Virology and Epidemiology, Baylor College of Medicine, Houston, TX 77030, United States
Metcalf, T.G., Department of Virology and Epidemiology, Baylor College of Medicine, Houston, TX 77030, United States
Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus
Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm 3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.
Scientific Publication
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