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פותח על ידי קלירמאש פתרונות בע"מ -
Immunological correspondence between arthropod hemocyanin subunits i. scorpion (leiurus, androctonus) and spider (eurypelma, cupiennius) hemocyanin
Year:
1984
Authors :
אבישר, אילנה
;
.
Volume :
365
Co-Authors:
Markla, J., Zoologisches Institut, Universität München, Germany
Gebauer, W., Zoologisches Institut, Universität München, Germany
Runzler, R., Zoologisches Institut, Universität München, Germany
Avissar, L., Department of Biochemistry, Tel-Aviv University, Germany
Facilitators :
From page:
619
To page:
632
(
Total pages:
14
)
Abstract:
1) The hemocyanins of the scorpions Leiwnis quinquestriatus and Androctonus australis, the tarantula Eurypelma califomicum (all 24-mers), and the lycosid spider Cupiennius salei (dode-camer) were dissociated into subunits, the sub-units isolated and studied by two-dimensional imrriunoelectrophoresis for interspecific cross-reactivities. 2) Androctonus hemocyanin yielded a pattern of 8 subunit types in agreement with data from Lamy et al. (1979, Arch. Biochem. Biophys. 193,140-149). Leiurus hemocyanin is also composed of 8 immunologically distinct subunits which could be assigned to the pattern of Androctonus in a subunit-to-subunit correlation. The subunit designations 1 to 6 of Lamy et al. could be adopted for both scorpion hemocyanins; however, in the present communication, Lamy's sub-units 3A/3B are designated as 3'/3”, because we could not unequivocally decide if 3’ = 3A and 3” =3B or vice versa. 3) The 7 subunit types a to g of Eurypelma hemocyanin could be correlated with the scorpion hemocyanin subunits as follows: a=3’, b = 5B, c = 3C,d = 5A,e = 6, f=2, g = 4. Additional cross-reactivities were detected between e/4, and f/5A, respectively. No subunit of Eurypelma hemocyanin is homologous to scorpion 3”, which could not be precipitated by anti-Eurypelma antiserum. 4) Antiserum against Cupiennius hemocyanin precipitated subunit f of Eurypelma and sub-units 2 and 5A of scorpion hemocyanin. 5) The published models of quaternary structure and a possible subunit phylogeny of arachnidan hemocyanins are discussed in view of the present results. Copyright © by Walter de Gruyter & Co.
Note:
Related Files :
Animal
Electrophoresis, Polyacrylamide Gel
immunoelectrophoresis
Peptide Fragments
polyacrylamide gel electrophoresis
Scorpions
עוד תגיות
תוכן קשור
More details
DOI :
10.1515/bchm2.1984.365.1.619
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21025
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Immunological correspondence between arthropod hemocyanin subunits i. scorpion (leiurus, androctonus) and spider (eurypelma, cupiennius) hemocyanin
365
Markla, J., Zoologisches Institut, Universität München, Germany
Gebauer, W., Zoologisches Institut, Universität München, Germany
Runzler, R., Zoologisches Institut, Universität München, Germany
Avissar, L., Department of Biochemistry, Tel-Aviv University, Germany
Immunological correspondence between arthropod hemocyanin subunits i. scorpion (leiurus, androctonus) and spider (eurypelma, cupiennius) hemocyanin
1) The hemocyanins of the scorpions Leiwnis quinquestriatus and Androctonus australis, the tarantula Eurypelma califomicum (all 24-mers), and the lycosid spider Cupiennius salei (dode-camer) were dissociated into subunits, the sub-units isolated and studied by two-dimensional imrriunoelectrophoresis for interspecific cross-reactivities. 2) Androctonus hemocyanin yielded a pattern of 8 subunit types in agreement with data from Lamy et al. (1979, Arch. Biochem. Biophys. 193,140-149). Leiurus hemocyanin is also composed of 8 immunologically distinct subunits which could be assigned to the pattern of Androctonus in a subunit-to-subunit correlation. The subunit designations 1 to 6 of Lamy et al. could be adopted for both scorpion hemocyanins; however, in the present communication, Lamy's sub-units 3A/3B are designated as 3'/3”, because we could not unequivocally decide if 3’ = 3A and 3” =3B or vice versa. 3) The 7 subunit types a to g of Eurypelma hemocyanin could be correlated with the scorpion hemocyanin subunits as follows: a=3’, b = 5B, c = 3C,d = 5A,e = 6, f=2, g = 4. Additional cross-reactivities were detected between e/4, and f/5A, respectively. No subunit of Eurypelma hemocyanin is homologous to scorpion 3”, which could not be precipitated by anti-Eurypelma antiserum. 4) Antiserum against Cupiennius hemocyanin precipitated subunit f of Eurypelma and sub-units 2 and 5A of scorpion hemocyanin. 5) The published models of quaternary structure and a possible subunit phylogeny of arachnidan hemocyanins are discussed in view of the present results. Copyright © by Walter de Gruyter & Co.
Scientific Publication
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