אסיף מאגר המחקר החקלאי

Pyrokinin/PBAN radio-receptor assay: Development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

Year:

2001

Source of publication :

Authors :

Volume :

22

Co-Authors:

Altstein, M., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Ben-Aziz, O., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Daniel, S., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Zeltser, I., Department of Organic Chemistry, Hebrew Univ. of Jerusalem, Jerusalem 91904, Israel
Gilon, C., Department of Organic Chemistry, Hebrew Univ. of Jerusalem, Jerusalem 91904, Israel

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Abstract:

A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (3H-tyrosyl-PBAN28-33NH2)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg27,D-Phe30]PBAN28-33NH2) PBAN antagonists with the receptor. © 2001 Elsevier Science Inc. All rights reserved.

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