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פותח על ידי קלירמאש פתרונות בע"מ -
A Myb-related transcription factor is involved in the phytochrome regulation of an arabidopsis Lhcb gene
Year:
1997
Source of publication :
Plant Cell
Authors :
קניגסבוך, דוד
;
.
Volume :
9
Co-Authors:
Wang, Z.-Y., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States
Kenigsbuch, D., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States
Sun, L., Life Sciences Department, Bar Ilan University, Ramat Gan, Israel
Harel, E., Life Sciences Department, Bar Ilan University, Ramat Gan, Israel
Ong, M.S., Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, United States
Tobin, E.M., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States, Department of Plant Sciences, Hebrew University, Jerusalem 91904, Israel
Facilitators :
From page:
491
To page:
507
(
Total pages:
17
)
Abstract:
We have isolated the gene for a protein designated CCA1. This protein can bind to a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb1*3, which is necessary for its regulation by phytochrome. The CCA1 protein interacted with two imperfect repeats in the Lhcb1*3 promoter, AA(A)/(c)AATCT, a sequence that is conserved in Lhcb genes. A region near the N terminus of CCA1, which has some homology to the repeated sequence found in the DNA binding domain of Myb proteins, is required for binding to the Lhcb1*3 promoter. Lines of transgenic Arabidopsis plants expressing antisense RNA for CCA1 showed reduced phytochrome induction of the endogenous Lhcb1*3 gene, whereas expression of another phytochrome-regulated gene, rbcS-1A, which encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, was not affected. Thus, the CCA1 protein acts as a specific activator of Lhcb1*3 transcription in response to brief red illumination. The expression of CCA1 RNA was itself transiently increased when etiolated seedlings were transferred to light. We conclude that the CCA1 protein is a key element in the functioning of the phytochrome signal transduction pathway leading to increased transcription of this Lhcb gene in Arabidopsis.
Note:
Related Files :
arabidopsis
CCA1 protein, Arabidopsis
Genetics
metabolism
molecular genetics
photosynthesis
עוד תגיות
תוכן קשור
More details
DOI :
10.1105/tpc.9.4.491
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21057
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
A Myb-related transcription factor is involved in the phytochrome regulation of an arabidopsis Lhcb gene
9
Wang, Z.-Y., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States
Kenigsbuch, D., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States
Sun, L., Life Sciences Department, Bar Ilan University, Ramat Gan, Israel
Harel, E., Life Sciences Department, Bar Ilan University, Ramat Gan, Israel
Ong, M.S., Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, United States
Tobin, E.M., Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095-1606, United States, Department of Plant Sciences, Hebrew University, Jerusalem 91904, Israel
A Myb-related transcription factor is involved in the phytochrome regulation of an arabidopsis Lhcb gene
We have isolated the gene for a protein designated CCA1. This protein can bind to a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb1*3, which is necessary for its regulation by phytochrome. The CCA1 protein interacted with two imperfect repeats in the Lhcb1*3 promoter, AA(A)/(c)AATCT, a sequence that is conserved in Lhcb genes. A region near the N terminus of CCA1, which has some homology to the repeated sequence found in the DNA binding domain of Myb proteins, is required for binding to the Lhcb1*3 promoter. Lines of transgenic Arabidopsis plants expressing antisense RNA for CCA1 showed reduced phytochrome induction of the endogenous Lhcb1*3 gene, whereas expression of another phytochrome-regulated gene, rbcS-1A, which encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, was not affected. Thus, the CCA1 protein acts as a specific activator of Lhcb1*3 transcription in response to brief red illumination. The expression of CCA1 RNA was itself transiently increased when etiolated seedlings were transferred to light. We conclude that the CCA1 protein is a key element in the functioning of the phytochrome signal transduction pathway leading to increased transcription of this Lhcb gene in Arabidopsis.
Scientific Publication
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