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Plant Science
Gupta, K., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Kayam, G., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Faigenboim-Doron, A., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Clevenger, J., Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, The University of Georgia, Tifton, GA, United States
Ozias-Akins, P., Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, The University of Georgia, Tifton, GA, United States
Hovav, R., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Pod-filling is an important stage of peanut (Arachis hypogaea) seed development. It is partially controlled by genetic factors, as cultivars considerably vary in pod-filling potential. Here, a study was done to detect changes in mRNA levels that accompany pod-filling processes. Four seed developmental stages were sampled from two peanut genotypes differing in their oil content and pod-filling potential. Transcriptome data were generated by RNA-Seq and explored with respect to genic and subgenomic patterns of expression. Very dynamic transcriptomic changes occurred during seed development in both genotypes. Yet, general higher expression rates of transcripts and an enrichment in processes involved "energy generation" and "primary metabolites" were observed in the genotype with the better pod-filling ("Hanoch"). A dataset of 584 oil-related genes was assembled and analyzed, resulting in several lipid metabolic processes highly expressed in Hanoch, including oil storage and FA synthesis/elongation. Homoeolog-specific gene expression analysis revealed that both subgenomes contribute to the oil genes expression. Yet, biases were observed in particular parts of the pathway with possible biological meaning, presumably explaining the genotypic variation in oil biosynthesis and pod-filling. This study provides baseline information and a resource that may be used to understand development and oil biosynthesis in the peanut seeds. © 2016 Elsevier Ireland Ltd.
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הספר "אוצר וולקני"
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תנאי שימוש
Gene expression profiling during seed-filling process in peanut with emphasis on oil biosynthesis networks
248
Gupta, K., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Kayam, G., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Faigenboim-Doron, A., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Clevenger, J., Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, The University of Georgia, Tifton, GA, United States
Ozias-Akins, P., Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, The University of Georgia, Tifton, GA, United States
Hovav, R., Department of Field Crops, Plant Sciences Institute, ARO, Bet-Dagan, Israel
Gene expression profiling during seed-filling process in peanut with emphasis on oil biosynthesis networks
Pod-filling is an important stage of peanut (Arachis hypogaea) seed development. It is partially controlled by genetic factors, as cultivars considerably vary in pod-filling potential. Here, a study was done to detect changes in mRNA levels that accompany pod-filling processes. Four seed developmental stages were sampled from two peanut genotypes differing in their oil content and pod-filling potential. Transcriptome data were generated by RNA-Seq and explored with respect to genic and subgenomic patterns of expression. Very dynamic transcriptomic changes occurred during seed development in both genotypes. Yet, general higher expression rates of transcripts and an enrichment in processes involved "energy generation" and "primary metabolites" were observed in the genotype with the better pod-filling ("Hanoch"). A dataset of 584 oil-related genes was assembled and analyzed, resulting in several lipid metabolic processes highly expressed in Hanoch, including oil storage and FA synthesis/elongation. Homoeolog-specific gene expression analysis revealed that both subgenomes contribute to the oil genes expression. Yet, biases were observed in particular parts of the pathway with possible biological meaning, presumably explaining the genotypic variation in oil biosynthesis and pod-filling. This study provides baseline information and a resource that may be used to understand development and oil biosynthesis in the peanut seeds. © 2016 Elsevier Ireland Ltd.
Scientific Publication
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