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פותח על ידי קלירמאש פתרונות בע"מ -
A heat treatment induced the expression of a Na+/H+ antiport gene (cNHX1) in citrus fruit
Year:
2002
Source of publication :
Plant Science
Authors :
בן חיים, גוזל
;
.
לוריא, סוזן
;
.
פבונצ'לו, דוד
;
.
פורת, רון
;
.
Volume :
162
Co-Authors:
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Pavoncello, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Ben-Hayyim, G., Department of Fruit Tree Breeding and Molecular Biology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lurie, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
957
To page:
963
(
Total pages:
7
)
Abstract:
In previous studies, we showed that a short hot water treatment (at 62 °C for 20 s) increased chilling tolerance in grapefruit. In order to gain more insight into the molecular mechanisms involved in heat-induced chilling tolerance responses, we performed cDNA differential display analysis and isolated a 600-bp polymerase chain reaction (PCR) fragment whose expression was enhanced 24 h after the heat treatment, and that showed high sequence homology with the 5′ region of various NHX1 vacuolar Na+/H+ antiporter genes. The full-length cDNA sequence of cNHX1 (for citrus NHX1) was further isolated by 3′-RACE; it had a total length of 2135 bp with an open reading frame of 1626 bp, and encoded a predicted polypetide of 542 amino acids with a molecular mass of 59.8 kDa. The cNHX1 protein shares 83, 78 and 56% identity with the other recently isolated Arabidopsis thaliana, Atriplex gmelini and Oryza sativa NHX1 proteins, respectively. It has been reported that the Arabidopsis AtNHX1 gene partially complemented a salt-sensitive yeast mutation and that overexpression of this gene significantly increased salt tolerance in transgenic plants. In the current study, we showed that the expression of the cNHX1 gene was temporarily induced by a heat treatment, and that its mRNA levels markedly increase during a 6-week cold storage period at 2 °C in heat-treated fruit but not in control untreated fruit, thus suggesting its possible involvement in heat-induced chilling tolerance responses. The expression of the cNHX1 gene was also markedly induced by salt stress, but not by other stresses, such as exposure to ethylene or UV irradiation. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Note:
Related Files :
arabidopsis
cold tolerance
DNA
ethylene
gene expression
grapefruits
proteins
salt stress
ultraviolet radiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0168-9452(02)00041-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21084
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
A heat treatment induced the expression of a Na+/H+ antiport gene (cNHX1) in citrus fruit
162
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Pavoncello, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Ben-Hayyim, G., Department of Fruit Tree Breeding and Molecular Biology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lurie, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
A heat treatment induced the expression of a Na+/H+ antiport gene (cNHX1) in citrus fruit
In previous studies, we showed that a short hot water treatment (at 62 °C for 20 s) increased chilling tolerance in grapefruit. In order to gain more insight into the molecular mechanisms involved in heat-induced chilling tolerance responses, we performed cDNA differential display analysis and isolated a 600-bp polymerase chain reaction (PCR) fragment whose expression was enhanced 24 h after the heat treatment, and that showed high sequence homology with the 5′ region of various NHX1 vacuolar Na+/H+ antiporter genes. The full-length cDNA sequence of cNHX1 (for citrus NHX1) was further isolated by 3′-RACE; it had a total length of 2135 bp with an open reading frame of 1626 bp, and encoded a predicted polypetide of 542 amino acids with a molecular mass of 59.8 kDa. The cNHX1 protein shares 83, 78 and 56% identity with the other recently isolated Arabidopsis thaliana, Atriplex gmelini and Oryza sativa NHX1 proteins, respectively. It has been reported that the Arabidopsis AtNHX1 gene partially complemented a salt-sensitive yeast mutation and that overexpression of this gene significantly increased salt tolerance in transgenic plants. In the current study, we showed that the expression of the cNHX1 gene was temporarily induced by a heat treatment, and that its mRNA levels markedly increase during a 6-week cold storage period at 2 °C in heat-treated fruit but not in control untreated fruit, thus suggesting its possible involvement in heat-induced chilling tolerance responses. The expression of the cNHX1 gene was also markedly induced by salt stress, but not by other stresses, such as exposure to ethylene or UV irradiation. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Scientific Publication
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